Evidence of proviral clearance following postpartum transmission of an ovine lentivirus

Lynn M Herrmann-Hoesing; Guy H Palmer; Donald P Knowles

doi:10.1016/j.virol.2006.12.021

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Evidence of proviral clearance following postpartum transmission of an ovine lentivirus

Journal article

Open access

Peer reviewed

Evidence of proviral clearance following postpartum transmission of an ovine lentivirus

Lynn M Herrmann-Hoesing, Guy H Palmer and Donald P Knowles

Virology (New York, N.Y.), Vol.362(1), pp.226-234

05/25/2007

DOI: https://doi.org/10.1016/j.virol.2006.12.021

Handle:

https://hdl.handle.net/2376/113083

PMID: 17267002

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Abstract

Sheep Diseases - transmission

Proviruses - isolation & purification

Enzyme-Linked Immunosorbent Assay

Sheep Diseases - virology

Milk - virology

DNA, Viral - analysis

RNA, Viral - blood

Postpartum Period

Infectious Disease Transmission, Vertical

Lentivirus Infections - transmission

Lentivirus Infections - virology

Antibodies, Viral - blood

Sequence Analysis, DNA

Lentivirus Infections - veterinary

Animals

Lentiviruses, Ovine-Caprine - isolation & purification

Polymerase Chain Reaction

Colostrum - virology

Female

Sheep

Disease Transmission, Infectious

Lentiviral transmission by transfer of infected colostrum and/or milk is considered to be highly efficient. In this study, postpartum transmission of ovine progressive pneumonia virus (OPPV) from 10 naturally infected ewes to their 23 lambs was followed from the perinatal period throughout a four-year period. The lambs were allowed to suckle from their dam from birth through 32 weeks of age. Virus was tracked by virus isolation, quantitative PCR (qPCR), and anti-OPPV antibody responses as measured by cELISA. Cell-associated OPPV was isolated from colostrum/milk cells in 7 out of 10 ewes and provirus envelope (env) loads ranged 8 to 10(5) copies/mug DNA in colostrum/milk cells from the 10 ewes using qPCR. Provirus env loads were also detected in the peripheral circulation of 21 lambs at 8 weeks and two lambs at 22 weeks. The qPCR product at 8 weeks was confirmed as the transmembrane (tm) gene of OPPV by cloning and sequencing. Both cELISA titers ranging from 325 to 3125 and cross-neutralizing antibody titers ranging from 6 to 162 to seven different OPPV strains were found in the colostrum of the 10 ewes. Furthermore, cELISA titers in serum from lambs remained detectable through 32 weeks following the clearance of provirus at 24 weeks. After 32 weeks, both provirus and anti-OPPV antibody responses have subsequently remained undetectable through 4 years of age. These data suggest the clearance of cell-associated lentiviruses from lamb circulation after passive transfer of antibody via colostrum.

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Title: Evidence of proviral clearance following postpartum transmission of an ovine lentivirus
Creators: Lynn M Herrmann-Hoesing - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, WA 99164-6630, USA. lherrman@vetmed.wsu.edu
Guy H Palmer
Donald P Knowles
Publication Details: Virology (New York, N.Y.), Vol.362(1), pp.226-234
Academic Unit: Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Publisher: United States
Identifiers: 99900548222501842
Language: English
Resource Type: Journal article