Journal article
Experimental infection of a bovine model with human isolates of Mycobacterium avium subsp. paratuberculosis
Veterinary immunology and immunopathology, Vol.141(3-4), pp.258-266
06/15/2011
Handle:
https://hdl.handle.net/2376/115425
PMCID: PMC3097062
PMID: 21477870
Abstract
Mycobacterium avium subsp. paratuberculosis (Map), the etiologic agent of Johne's disease (JD) in ruminants, has been implicated in the pathogenesis of Crohn's disease (CD) in humans. We developed a bovine ileal cannulation model to facilitate comparison of the immune response to Map and the mechanisms of pathogenesis in cattle and humans. Initial studies showed a T cannula could be maintained for up to a year in calves without inducing inflammation or adversely affecting intestinal function. Map introduced through the cannula established a persistent low level of infection without inflammation. Infection elicited an immune response to Map antigens detectable by flow cytometry. Further studies now show the cannulation model can be used with cows during the later stage of infection, affording access to the target tissue at all stages of infection. The studies also revealed no difference in infectivity or immunogenicity of isolates of Map obtained from cattle or humans with CD. Comparison of the immune response to Map during the early and late stages of infection using PCR, flow cytometry and QRT-PCR, showed the immune response early in the disease process is dominated by CD4 T cells. A CD8 response is delayed but comparable at later stages of infection. Genes for pro-inflammatory cytokines IFN-γ and the recently identified genes encoding IL-17 and IL-22 are up regulated in infected animals. These findings reveal that both human and bovine isolates of Map can establish infection and induce similar immune responses in a bovine model. They also reveal the cytokine responses elicited in cattle are similar to those implicated in CD pathogenesis.
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Details
- Title
- Experimental infection of a bovine model with human isolates of Mycobacterium avium subsp. paratuberculosis
- Creators
- Andrew J Allen - Department of Veterinary Clinical Sciences, Washington State University, Pullman, WA 99164, USAKun-Taek Park - Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USAGeorge M Barrington - Department of Veterinary Clinical Sciences, Washington State University, Pullman, WA 99164, USAKevin K Lahmers - Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USAGaber S Abdellrazeq - Department of Microbiology, Faculty of Veterinary Med, Alexandria University, EgyptHeba M Rihan - Department of Bacteriology, Mycology and Immunology, Faculty of Vet Med, Mansoura University, EgyptSrinand Sreevatsan - Veterinary Population Medicine Department, University of Minnesota, St. Paul, MN 55108, USAChristopher Davies - Department of Animal, Dairy and Veterinary Sciences and Center for Integrated BioSystems, Utah State University, Logan, UT 84322, USAMary J Hamilton - Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USAWilliam C Davis - Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164, USA
- Publication Details
- Veterinary immunology and immunopathology, Vol.141(3-4), pp.258-266
- Academic Unit
- Veterinary Clinical Sciences, Department of; Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier B.V
- Identifiers
- 99900547654801842
- Language
- English
- Resource Type
- Journal article