Exposure to nitrous oxide stimulates a nitric oxide-dependent neuronal release of β-endorphin in ventricular-cisternally-perfused rats

Lisa M Zelinski; Yusuke Ohgami; Raymond M Quock

doi:10.1016/j.brainres.2009.08.091

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Exposure to nitrous oxide stimulates a nitric oxide-dependent neuronal release of β-endorphin in ventricular-cisternally-perfused rats

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Exposure to nitrous oxide stimulates a nitric oxide-dependent neuronal release of β-endorphin in ventricular-cisternally-perfused rats

Lisa M Zelinski, Yusuke Ohgami and Raymond M Quock

Brain research, Vol.1300, pp.37-40

2009

DOI: https://doi.org/10.1016/j.brainres.2009.08.091

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https://hdl.handle.net/2376/115367

PMCID: PMC2783921

PMID: 19747467

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Abstract

Nitrous oxide

β-endorphin

Rat

Ventricular-cisternal perfusion

Nitric oxide

We have previously shown that the antinociceptive effect of nitrous oxide (N 2O) in the rat hot plate test is sensitive to antagonism by antisera against the endogenous opioid peptide β-endorphin. Moreover, N 2O-induced antinociception is reduced by inhibition of nitric oxide (NO) production in the brain. To test the hypothesis that N 2O might stimulate an NO-dependent neuronal release of β-endorphin, we conducted a ventricular-cisternal perfusion with artificial cerebrospinal fluid (aCSF) in urethane-anesthetized Sprague–Dawley rats. Ten-minute fractions of aCSF perfusate were collected from separate groups of room air-exposed rats, N 2O-exposed rats, and L-NAME-pretreated, N 2O-exposed rats; they were then analyzed for their content of NO metabolites and β-endorphin. Compared to room air control, exposure to 70% N 2O increased perfusate levels of the NO metabolites nitrite and nitrate as well as β-endorphin. Pretreatment of rats with L-N G-nitro arginine methyl ester, an inhibitor of NO synthase, prevented the N 2O-induced increases in nitrite, nitrate and β-endorphin. These findings demonstrate in an in vivo rat model that N 2O may stimulate an NO-dependent neuronal release of β-endorphin.

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Title: Exposure to nitrous oxide stimulates a nitric oxide-dependent neuronal release of β-endorphin in ventricular-cisternally-perfused rats
Creators: Lisa M Zelinski - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164, USA
Yusuke Ohgami - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164, USA
Raymond M Quock - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164, USA
Publication Details: Brain research, Vol.1300, pp.37-40
Academic Unit: Psychology, Department of
Publisher: Elsevier B.V
Identifiers: 99900547459901842
Language: English
Resource Type: Journal article