Journal article
Expression and immune recognition of the conserved MSP4 outer membrane protein of Anaplasma marginale
Infection and immunity, Vol.61(12), pp.5245-5251
12/1993
Handle:
https://hdl.handle.net/2376/107924
PMCID: PMC281308
PMID: 7693596
Abstract
Defining conserved, protective epitopes is essential to the design of an effective vaccine against bovine anaplasmosis. MSP4, one of six initial body proteins recognized by a neutralizing serum, is conserved among Anaplasma marginale isolates at both the protein and the DNA levels. Sera from cattle immunized with an outer membrane fraction of A. marginale and protected from a virulent challenge bind MSP4. The gene for MSP4 has been cloned, and the recombinant protein has been expressed, isolated, and demonstrated to share epitopes with the native protein expressed on initial bodies. MSP4 may have a greater potential to protect cattle from a challenge by heterologous isolates than other A. marginale surface proteins, which vary widely in size and structure.
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Details
- Title
- Expression and immune recognition of the conserved MSP4 outer membrane protein of Anaplasma marginale
- Creators
- S M Oberle - Department of Infectious Diseases, University of Florida, Gainesville 32611-0880G H Palmer - Department of Infectious Diseases, University of Florida, Gainesville 32611-0880A F Barbet - Department of Infectious Diseases, University of Florida, Gainesville 32611-0880
- Publication Details
- Infection and immunity, Vol.61(12), pp.5245-5251
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Identifiers
- 99900546935001842
- Language
- English
- Resource Type
- Journal article