Journal article
Expression and regulation of phospholipase D isoforms in mammalian cell lines
Journal of cellular physiology, Vol.182(1), pp.77-87
01/2000
Handle:
https://hdl.handle.net/2376/115477
PMID: 10567919
Abstract
Phospholipase D (PLD) is activated in mammalian cells in response to diverse stimuli that include growth factors, activators of protein kinase C, and agonists binding to G‐protein‐coupled receptors. Two forms of mammalian PLD, PLD1 and PLD2, have been identified. Expression of mRNA and protein for PLD1 and PLD2 was analyzed in the following cell lines: A7r5 (rat vascular smooth muscle); EL4 (mouse thymoma); HL‐60 (human myeloid leukemia); Jurkat (human leukemia); PC‐3 (human prostate adenocarcinoma); PC‐12K (rat phaeochromocytoma); and Rat‐1 HIR (rat fibroblast). All, with the exception of EL4, express agonist‐activated PLD activity. PLD1 is expressed in A7r5, HL‐60, PC‐3, and Rat‐1, while PLD2 is expressed in A7r5, Jurkat, PC12K, PC‐3, and Rat‐1. Neither isoform is expressed in EL4. Guanine nucleotide‐independent PLD activity is present in membranes from all cells expressing PLD2. In PC12K cells, which express only PLD2, treatment with nerve growth factor causes neurite outgrowth and increases expression of PLD2 mRNA and protein within 6–12 h. A corresponding increase is observed in membrane PLD activity and in phorbol‐12‐myristate‐13‐acetate (PMA)‐stimulated PLD activity in intact cells. These results show that PLD2 can be regulated both pretranslationally and posttranslationally by agonists. J. Cell. Physiol. 182:77–87, 2000. © 2000 Wiley‐Liss, Inc.
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Details
- Title
- Expression and regulation of phospholipase D isoforms in mammalian cell lines
- Creators
- Terra C GibbsKathryn E Meier
- Publication Details
- Journal of cellular physiology, Vol.182(1), pp.77-87
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- John Wiley & Sons, Inc; New York
- Number of pages
- 11
- Grant note
- University Research Committee, Medical University of South Carolina UNCF/Merck Science Research Dissertation Fellowship U.S. Department of Defense (DAMD17‐98‐8524) National Science Foundation (EPS‐9630167)
- Identifiers
- 99900547519201842
- Language
- English
- Resource Type
- Journal article