Journal article
Expression of Anaplasma marginale Ankyrin Repeat-Containing Proteins during Infection of the Mammalian Host and Tick Vector
Infection and immunity, Vol.79(7), pp.2847-2855
07/2011
Handle:
https://hdl.handle.net/2376/104132
PMCID: PMC3191954
PMID: 21576345
Abstract
Transmission of tick-borne pathogens requires transition between distinct host environments with infection and replication in host-specific cell types.
Anaplasma marginale
illustrates this transition: in the mammalian host, the bacterium infects and replicates in mature (nonnucleated) erythrocytes, while in the tick vector, replication occurs in nucleated epithelial cells. We hypothesized that proteins containing ankyrin motifs would be expressed by
A. marginale
only in tick cells and would traffic to the infected host cell nucleus.
A. marginale
encodes three proteins containing ankyrin motifs, an AnkA orthologue (the AM705 protein), AnkB (the AM926 protein), and AnkC (the AM638 protein). All three
A. marginale
Anks were confirmed to be expressed during intracellular infection: AnkA is expressed at significantly higher levels in erythrocytes, AnkB is expressed equally by both infected erythrocytes and tick cells, and AnkC is expressed exclusively in tick cells. There was no evidence of any of the Ank proteins trafficking to the nucleus. Thus, the hypothesis that ankyrin-containing motifs were predictive of cell type expression and nuclear localization was rejected. In contrast, AnkA orthologues in the closely related
A. phagocytophilum
and
Ehrlichia chaffeensis
have been shown to localize to the host cell nucleus. This difference, together with the lack of a nuclear localization signal in any of the AnkA orthologues, suggests that trafficking may be mediated by a separate transporter rather than by endogenous signals. Selection for divergence in Ank function among
Anaplasma
and
Ehrlichia
spp. is supported by both locus and allelic analyses of genes encoding orthologous proteins and their ankyrin motif compositions.
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Details
- Title
- Expression of Anaplasma marginale Ankyrin Repeat-Containing Proteins during Infection of the Mammalian Host and Tick Vector
- Creators
- Solomon S Ramabu - Program in Vector-borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164David A Schneider - Animal Diseases Research Unit, USDA-ARS, Pullman, Washington 99164Kelly A Brayton - Program in Vector-borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164Massaro W Ueti - Animal Diseases Research Unit, USDA-ARS, Pullman, Washington 99164Telmo Graça - Program in Vector-borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164James E Futse - Program in Vector-borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164Susan M Noh - Animal Diseases Research Unit, USDA-ARS, Pullman, Washington 99164Timothy V Baszler - Program in Vector-borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164Guy H Palmer - Program in Vector-borne Diseases, Department of Veterinary Microbiology and Pathology, and The Paul G. Allen School for Global Animal Health, Washington State University, Pullman, Washington 99164
- Publication Details
- Infection and immunity, Vol.79(7), pp.2847-2855
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Identifiers
- 99900546975801842
- Language
- English
- Resource Type
- Journal article