Journal article
Expression of Anaplasma marginale Major Surface Protein 2 Operon-Associated Proteins during Mammalian and Arthropod Infection
Infection and immunity, Vol.70(11), pp.6005-6012
11/2002
Handle:
https://hdl.handle.net/2376/101604
PMCID: PMC130398
PMID: 12379676
Abstract
The antigenically variant major surface protein 2 (MSP2) of
Anaplasma marginale
is expressed from a 3.5-kb operon that contains, in a 5′-to-3′ direction, four open reading frames,
opag3
,
opag2
,
opag1
, and
msp2
. This operon structure was shown to be conserved among genotypically and phenotypically distinct
A. marginale
,
A. ovis
, and
A. centrale
strains
.
The individual OpAG amino acid sequences are highly conserved among
A. marginale
strains, with identities ranging from 95 to 99%. OpAG2 and OpAG3 were expressed by all examined
A. marginale
strains during the acute rickettsemia in the mammalian host and, like MSP2, localize to the bacterial surface. OpAG2 and OpAG3 were also expressed in an infected
Ixodes scapularis
tick cell line. In contrast, the same
A. marginale
strains expressed only OpAG2 in two different
Dermacentor
spp. during transmission feeding. OpAG1 expression was not detected in the infected mammalian host, the infected tick cell line, or within infected
Dermacentor
ticks. The differential expression of outer membrane proteins from within an operon is a novel finding in tick-transmitted bacteria, and the regulation of expression may be broadly applicable to understanding how the pathogen adapts to the mammalian host-tick vector transition.
Metrics
7 Record Views
Details
- Title
- Expression of Anaplasma marginale Major Surface Protein 2 Operon-Associated Proteins during Mammalian and Arthropod Infection
- Creators
- Christiane V Löhr - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Kelly A Brayton - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Varda Shkap - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Thea Molad - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Anthony F Barbet - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Wendy C Brown - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Guy H Palmer - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040
- Publication Details
- Infection and immunity, Vol.70(11), pp.6005-6012
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546638301842
- Language
- English
- Resource Type
- Journal article