Journal article
Expression of Anaplasma marginale Major Surface Protein 2 Variants during Persistent Cyclic Rickettsemia
Infection and immunity, Vol.66(3), pp.1200-1207
03/1998
Handle:
https://hdl.handle.net/2376/115706
PMCID: PMC108034
PMID: 9488414
Abstract
Anaplasma marginale
is an intraerythrocytic rickettsial pathogen of cattle in which infection persists for the life of the animal. Persistent
A. marginale
infection is characterized by repetitive rickettsemic cycles which we hypothesize reflect emergence of
A. marginale
antigenic variants. In this study, we determined whether variants of major surface protein 2 (MSP-2), a target of protective immunity encoded by a polymorphic multigene family, arise during persistent rickettsemia. By using a quantitative competitive PCR to identify rickettsemic cycles,
msp-2
transcripts expressed in vivo were isolated from peak rickettsemia of sequential cycles. Cloning and sequencing of
msp-2
cDNA revealed that genetic variants of MSP-2 emerge representing a minimum of four genetic variant types in each cycle during persistent infection. Two-color immunofluorescence using variant-specific antibody showed that emergence of MSP-2 variants resulted in expression of a minimum of three antigenic types of MSP-2 within one rickettsemic cycle. Therefore immune control of each cycle would require responses to an antigenically diverse
A. marginale
population. These findings demonstrate that polymorphic MSP-2 variants emerge during cyclic rickettsemia in persistent
A. marginale
infection and suggest that emergent variants play an important role in persistence.
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Details
- Title
- Expression of Anaplasma marginale Major Surface Protein 2 Variants during Persistent Cyclic Rickettsemia
- Creators
- Dorothy M French - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Terry F McElwain - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Travis C McGuire - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Guy H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164
- Publication Details
- Infection and immunity, Vol.66(3), pp.1200-1207
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547820401842
- Language
- English
- Resource Type
- Journal article