Journal article
Expression of Arabidopsis callose synthase 5 results in callose accumulation and cell wall permeability alteration
Plant science (Limerick), Vol.183, pp.1-8
2012
Handle:
https://hdl.handle.net/2376/105836
PMID: 22195570
Abstract
[Display omitted]
► We expressed GFP-CalS5 in tobacco BY-2 cells. ► GFP-CalS5 is localized to the plasma membrane and endomembranes. ► Callose is accumulated in the cell wall, but absent in the endomembranes. ► Water permeability and strength of the cell wall is changed.
Callose is the major polysaccharide present in the callose wall of developing microspores and the growing pollen tube wall. It is also an essential component of other specialized cell walls and its synthesis can be induced by pathogen infection, wounding and environmental cues. Among the 12 callose synthase genes (
CalS) present in the
Arabidopsis genome,
CalS5 plays the predominant role in the synthesis of the callose wall, callose plugs and pollen tube wall. When expressed as a GFP-tagged protein in cultured tobacco BY-2 cells, CalS5 was found to be present in the plasma membrane and the Golgi-related endomembranes. Unlike the cell plate-specific CalS1 isozyme, CalS5 was not concentrated to the cell plate at cytokinesis. Expression of CalS5 resulted in callose accumulation only in the cell wall of BY-2 cells. The fact that no callose was found in the endomembranes suggests that CalS5 is not functional in that compartment. These cells exhibited a decreased plasmolysis rate in hypotonic solutions and an increased cytolysis rate in hypertonic conditions. This study demonstrates that an artificial callose wall could be synthesized by expressing a callose synthase enzyme.
Metrics
10 Record Views
Details
- Title
- Expression of Arabidopsis callose synthase 5 results in callose accumulation and cell wall permeability alteration
- Creators
- Bo Xie - Department of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844, USAYunfei Deng - Department of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844, USAMasahiro M Kanaoka - Department of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844, USAKiyotaka Okada - Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto 606-8502, JapanZonglie Hong - Department of Plant, Soil, and Entomological Sciences and Program of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844, USA
- Publication Details
- Plant science (Limerick), Vol.183, pp.1-8
- Academic Unit
- Center for Reproductive Biology
- Publisher
- Elsevier Ireland Ltd
- Identifiers
- 99900546992101842
- Language
- English
- Resource Type
- Journal article