Journal article
Expression of Equi Merozoite Antigen 2 during Development of Babesia equi in the Midgut and Salivary Gland of the Vector Tick Boophilus microplus
Journal of clinical microbiology, Vol.41(12), pp.5803-5809
12/2003
Handle:
https://hdl.handle.net/2376/110850
PMCID: PMC308990
PMID: 14662988
Abstract
Equi merozoite antigens 1 and 2 (EMA-1 and EMA-2) are
Babesia equi
proteins expressed on the parasite surface during infection in horses and are orthologues of proteins in
Theileria
spp., which are also tick-transmitted protozoal pathogens. We determined in this study whether EMA-1 and EMA-2 were expressed within the vector tick
Boophilus microplus. B. equi
transitions through multiple, morphologically distinct stages, including sexual stages, and these transitions culminate in the formation of infectious sporozoites in the tick salivary gland. EMA-2-positive
B. equi
stages in the midgut lumen and midgut epithelial cells of
Boophilus microplus
nymphs were identified by reactivity with monoclonal antibody 36/253.21. This monoclonal antibody also recognized
B. equi
in salivary glands of adult
Boophilus microplus
. In addition, quantification of
B. equi
in the mammalian host and vector tick indicated that the duration of tick feeding and parasitemia levels affected the percentage of nymphs that contained morphologically distinct
B. equi
organisms in the midgut. In contrast, there was no conclusive evidence that
B. equi
EMA-1 was expressed in either the
Boophilus microplus
midgut or salivary gland when monoclonal antibody 36/18.57 was used. The expression of
B. equi
EMA-2 in
Boophilus microplus
provides a marker for detecting the various development stages and facilitates the identification of novel stage-specific
Babesia
proteins for testing transmission-blocking immunity.
Metrics
7 Record Views
Details
- Title
- Expression of Equi Merozoite Antigen 2 during Development of Babesia equi in the Midgut and Salivary Gland of the Vector Tick Boophilus microplus
- Creators
- Massaro W Ueti - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Guy H Palmer - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Lowell S Kappmeyer - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Glen A Scoles - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040Donald P Knowles - Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040
- Publication Details
- Journal of clinical microbiology, Vol.41(12), pp.5803-5809
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547275701842
- Language
- English
- Resource Type
- Journal article