Journal article
Expression of Immunoglobulin G1 Receptors by Bovine Mammary Epithelial Cells and Mammary Leukocytes
Journal of dairy science, Vol.80(1), pp.86-93
01/1997
Handle:
https://hdl.handle.net/2376/109878
PMID: 9120099
Abstract
The objective of this study was to identify and evaluate expression of IgG1 receptors by different cell types in mammary tissue sections and digest-dispersed cells from the bovine mammary gland. An immunohistochemical system utilizing avidin-biotin-peroxidase complex demonstrated epithelial expression of IgG1 receptors in mammary tissue sections from cows producing colostrum but not from cows in lactation. Fluorescence flow cytometry demonstrated that cells dispersed in digests from both tissues producing colostrum and lactating tissues selectively bound IgG1. Fluorescence flow cytometry, using monoclonal antibodies to cell surface molecules, cytokeratin, and IgG1 revealed that leukocytes constituted the largest percentage of cells and were the predominant cell type binding IgG1 in mammary tissue digests. Although IgG1 binding to epithelial cells predominated in the gland during colostrum production in situ, digestion and filtration to produce single cell suspensions resulted in the loss of large numbers of epithelial cells. Studies of Ig binding of cells produced by enzymatic digestion must account for the types of cells surviving the digestion process.
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Details
- Title
- Expression of Immunoglobulin G1 Receptors by Bovine Mammary Epithelial Cells and Mammary Leukocytes
- Creators
- G.M Barrington - Washington State University, Pullman 99164T.E Besser - Washington State University, Pullman 99164W.C Davis - Washington State University, Pullman 99164C.C Gay - Washington State University, Pullman 99164J.J Reeves - Washington State University, Pullman 99164T.B McFadden - Ruakura Agricultural Centre, Hamilton, New Zealand
- Publication Details
- Journal of dairy science, Vol.80(1), pp.86-93
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547179701842
- Language
- English
- Resource Type
- Journal article