Expression of Mirlet7 family microRNAs in response to retinoic acid-induced spermatogonial differentiation in mice

Ming-Han Tong; Debra Mitchell; Ryan Evanoff; Michael D Griswold

doi:10.1095/biolreprod.110.089458

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Expression of Mirlet7 family microRNAs in response to retinoic acid-induced spermatogonial differentiation in mice

Journal article

Open access

Peer reviewed

Expression of Mirlet7 family microRNAs in response to retinoic acid-induced spermatogonial differentiation in mice

Ming-Han Tong, Debra Mitchell, Ryan Evanoff and Michael D Griswold

Biology of reproduction, Vol.85(1), pp.189-197

07/2011

DOI: https://doi.org/10.1095/biolreprod.110.089458

Handle:

https://hdl.handle.net/2376/103982

PMID: 21430230

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Abstract

RNA Processing, Post-Transcriptional

Mice, Inbred C57BL

Cells, Cultured

Male

MicroRNAs - metabolism

Receptors, Retinoic Acid - metabolism

Spermatogonia - metabolism

Animals

Cell Differentiation

Mice

Spermatogonia - cytology

RNA-Binding Proteins - metabolism

Tretinoin

Spermatogonial differentiation is orchestrated by the precise control of gene expression involving retinoic acid signaling. MicroRNAs have emerged as important regulators of spermatogenesis, and here we show that the Mirlet7 family miRNAs are expressed in mouse spermatogonia and spermatocytes. Retinoic acid significantly leads to the induction of Mirlet7 miRNAs through suppression of Lin28. We further confirmed both in vitro and in vivo that expressions of Mycn, Ccnd1, and Col1a2, which are targets of Mirlet7, were downregulated during spermatogonial differentiation. These results suggest that Mirlet7 family miRNAs play a role in retinoic acid-induced spermatogonial differentiation.

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Title: Expression of Mirlet7 family microRNAs in response to retinoic acid-induced spermatogonial differentiation in mice
Creators: Ming-Han Tong - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164, USA
Debra Mitchell
Ryan Evanoff
Michael D Griswold
Publication Details: Biology of reproduction, Vol.85(1), pp.189-197
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: R37 HD010808 / NICHD NIH HHS R01 HD010808 / NICHD NIH HHS R21 HD067775 / NICHD NIH HHS R21 HD067775-01A1 / NICHD NIH HHS R21 HD067775-02 / NICHD NIH HHS HD10808 / NICHD NIH HHS
Identifiers: 99900546822401842
Language: English
Resource Type: Journal article