Journal article
Expression of callose synthase genes and its connection with Npr1 signaling pathway during pathogen infection
Planta, Vol.229(1), pp.87-98
12/2008
Handle:
https://hdl.handle.net/2376/113272
PMID: 18807070
Abstract
Callose synthesis occurs at specific stages of plant cell wall development in all cell types, and in response to pathogen attack, wounding and physiological stresses. We determined the expression pattern of “upstream regulatory sequence” of 12 Arabidopsis callose synthase genes (CalS1–12) genes and demonstrated that different callose synthases are expressed specifically in different tissues during plant development. That multiple CalS genes are expressed in the same cell type suggests the possibility that CalS complex may be constituted by heteromeric subunits. Five CalS genes were induced by pathogen (Hyaloperonospora arabidopsis, previously known as Peronospora parasitica, the causal agent of downy mildew) or salicylic acid (SA), while the other seven CalS genes were not affected by these treatments. Among the genes that are induced, CalS1 and CalS12 showed the highest responses. In Arabidopsis
npr1 mutant, impaired in response of pathogenesis related (PR) genes to SA, the induction of CalS1 and CalS12 genes by the SA or pathogen treatments was significantly reduced. The patterns of expression of the other three CalS genes were not changed significantly in the npr1 mutant. These results suggest that the high induction observed of CalS1 and CalS12 is Npr1 dependent while the weak induction of five CalS genes is Npr1 independent. In a T-DNA knockout mutant of CalS12, callose encasement around the haustoria on the infected leaves was reduced and the mutant was found to be more resistant to downy mildew as compared to the wild type plants.
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Details
- Title
- Expression of callose synthase genes and its connection with Npr1 signaling pathway during pathogen infection
- Creators
- Xiaoyun Dong - The Children’s Hospital of Philadelphia 34th st. and Civic Center Blvd. ARC/314G Philadelphia PA 19104 USAZonglie Hong - Department of Microbiology, Molecular Biology and Biochemistry University of Idaho Moscow ID 83844-3052 USAJayanta Chatterjee - Plant Biotechnology Center and Department of Molecular Genetics The Ohio State University 240 Rightmire Hall, 1060 Carmack Road Columbus OH 43210-1002 USASunghan Kim - Plant Biotechnology Center and Department of Molecular Genetics The Ohio State University 240 Rightmire Hall, 1060 Carmack Road Columbus OH 43210-1002 USADesh Verma - Plant Biotechnology Center, Department of Plant Pathology and Department of Molecular Genetics The Ohio State University 240 Rightmire Hall, 1060 Carmack Road Columbus OH 43210-1002 USA
- Publication Details
- Planta, Vol.229(1), pp.87-98
- Academic Unit
- Center for Reproductive Biology
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900547798101842
- Language
- English
- Resource Type
- Journal article