Journal article
Expression of pigmentation genes following electroporation of albino Monascus purpureus
Journal of industrial microbiology & biotechnology, Vol.30(6), pp.369-374
06/2003
Handle:
https://hdl.handle.net/2376/115837
PMID: 12750945
Abstract
A UV-induced albino strain of Monascus purpureus was subjected to electroporation in the presence of genomic DNA from a wild-type red strain of the fungus. Eight colonies expressed color after several weeks of growth. The growth rates of all eight color variants were significantly greater than the recipient and donor strains under some culture conditions. Spectrophotometric analysis of the pigments extracted from the color variants revealed the pigments had absorbance spectra different from the DNA donor strain. These color variants may have resulted from transformation with wild-type DNA, mutation reversion, or activation of alternative pathway(s)—i.e., new mutations—that resulted in pigment production.
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Details
- Title
- Expression of pigmentation genes following electroporation of albino Monascus purpureus
- Creators
- K Lakrod - Mission College Muak Lek Saraburi ThailandC Chaisrisook - Department of Microbiology, Faculty of Science Kasetsart University Bangkok ThailandD Skinner - USDA-ARS and Department of Crop and Soil Science Washington State University Pullman WA 99164–6420 USA
- Publication Details
- Journal of industrial microbiology & biotechnology, Vol.30(6), pp.369-374
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900547850001842
- Language
- English
- Resource Type
- Journal article