Journal article
Expression of progesterone receptor membrane component 1 and its partner serpine 1 mRNA binding protein in uterine and placental tissues of the mouse and human
Molecular and cellular endocrinology, Vol.287(1), pp.81-89
2008
Handle:
https://hdl.handle.net/2376/105638
PMID: 18440126
Abstract
Although activation of the nuclear progesterone (P
4) receptor (PGR) is required for uterine function, some of the actions of P
4 are mediated through a PGR-independent mechanism. The receptors that account for the PGR-independent actions have not been identified with certainty. The purpose of this study was to assess the expression, localization and hormonal regulation of two novel P
4 receptor candidates, P
4 receptor membrane component (PGRMC) 1 and PGRMC2, as well as the PGRMC1 partner Serpine 1 mRNA binding protein (SERBP1). Unlike
Pgrmc1 and
Serbp1, which remained unchanged throughout the estrous cycle,
Pgrmc2 was highly up-regulated during proestrus and metestrus. Immunohistochemical analyses suggest that PGRMC1 and SERBP1 promote differentiation, since the expression of these proteins increased in endometrial cells undergoing steroid-depended terminal differentiation. Progesterone rather than estrogen appears to be primarily responsible for up-regulating the expression of PGRMCs. PGRMC1 and SERBP1 also showed overlapping patterns of expression in the human placenta and associated membranes with the most abundant expression in smooth muscle of the placental vasculature, villous capillaries and the syncytiotrophoblast. Based on these findings, it is proposed that PGRMC1:SERBP1 protein complex functions in events important to early pregnancy including cellular differentiation, modulation of apoptosis and steroidogenesis. These studies provide a platform from which to build a clearer understanding of P
4 actions in the female reproductive tract and placental tissues that are mediated by non-classical mechanisms.
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Details
- Title
- Expression of progesterone receptor membrane component 1 and its partner serpine 1 mRNA binding protein in uterine and placental tissues of the mouse and human
- Creators
- Ling Zhang - Vincent Center for Reproductive Biology, Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, United StatesYoshiaki Kanda - Vincent Center for Reproductive Biology, Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, United StatesDrucilla J Roberts - Department of Pathology, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, United StatesJeffrey L Ecker - Vincent Center for Reproductive Biology, Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, United StatesRalf Losel - Faculty of Clinical Medicine, Clinical Pharmacology Mannheim, University of Heidelberg, D-68135 Mannheim, GermanyMartin Wehling - Faculty of Clinical Medicine, Clinical Pharmacology Mannheim, University of Heidelberg, D-68135 Mannheim, GermanyJohn J Peluso - Departments of Cell Biology and Obstetrics and Gynecology, University of Connecticut Health Center, Farmington, CT 06030, United StatesJames K Pru - Vincent Center for Reproductive Biology, Vincent Obstetrics and Gynecology Service, Massachusetts General Hospital/Harvard Medical School, Boston, MA 02114, United States
- Publication Details
- Molecular and cellular endocrinology, Vol.287(1), pp.81-89
- Academic Unit
- Animal Sciences, Department of
- Publisher
- Elsevier Ireland Ltd
- Identifiers
- 99900546746501842
- Language
- English
- Resource Type
- Journal article