Journal article
Expression of the oligopeptide transporter, PepT1, in larval Atlantic cod ( Gadus morhua)
Comparative Biochemistry and Physiology, Part B, Vol.150(2), pp.177-182
2008
Handle:
https://hdl.handle.net/2376/101442
PMID: 18396432
Abstract
The intestinal absorption of di- and tri-peptides generally occurs via the oligopeptide transporter, PepT1. This study evaluates the expression of PepT1 in larval Atlantic cod (
Gadus morhua) during the three weeks following the onset of exogenous feeding. Larval Atlantic cod were fed either wild captured zooplankton or enriched rotifers. cDNA was prepared from whole cod larvae preceding first feeding and at 1000 each Tuesday and Thursday for the following three weeks. Spatial and temporal expression patterns of PepT1 mRNA were compared between fish consuming the two prey types using
in situ hybridization and quantitative real-time PCR. Results indicated that PepT1 mRNA was expressed prior to the onset of exogenous feeding. In addition, PepT1 was expressed throughout the digestive system except the esophagus and sphincter regions. Expression slightly increased following first-feeding and continued to increase throughout the study for larvae feeding on both prey types. When comparing PepT1 expression in larvae larger than 0.15-mg dry mass with expression levels in larvae prior to feeding, no differences were detected for larvae fed rotifers, but the larvae fed zooplankton had significantly greater PepT1 expression at the larger size. In addition, PepT1 expression in the zooplankton fed larvae larger than 0.15-mg dry mass had significantly greater expression than rotifer fed larvae of a similar weight. Switching prey types did not affect PepT1 expression. These results indicate that Atlantic cod PepT1 expression was slightly different relative to dietary treatment during the three weeks following first-feeding. In addition, PepT1 may play an important role in the larval nutrition since it is widely expressed in the digestive tract.
Metrics
10 Record Views
Details
- Title
- Expression of the oligopeptide transporter, PepT1, in larval Atlantic cod ( Gadus morhua)
- Creators
- J.J Amberg - University of Idaho, Hagerman Fish Culture Experiment Station, 3059-F National Fish Hatchery Road, Hagerman, ID 83332, USAC Myr - Department of Biology, University of Bergen. Pb 7800, 5020 Bergen, NorwayY Kamisaka - Department of Biology, University of Bergen. Pb 7800, 5020 Bergen, NorwayA.-E.O Jordal - Department of Biology, University of Bergen. Pb 7800, 5020 Bergen, NorwayM.B Rust - Northwest Fisheries Science Center, Resource Enhancement and Utilization Technologies Division, 2725 Montlake Blvd E, Seattle, WA 98112, USAR.W Hardy - University of Idaho, Hagerman Fish Culture Experiment Station, 3059-F National Fish Hatchery Road, Hagerman, ID 83332, USAR Koedijk - Department of Biology, University of Bergen. Pb 7800, 5020 Bergen, NorwayI Rønnestad - Department of Biology, University of Bergen. Pb 7800, 5020 Bergen, Norway
- Publication Details
- Comparative Biochemistry and Physiology, Part B, Vol.150(2), pp.177-182
- Academic Unit
- UNKNOWN
- Publisher
- Elsevier Inc
- Identifiers
- 99900546791001842
- Language
- English
- Resource Type
- Journal article