Journal article
Expression of β-Galactosidase in Preimplantation Ovine and Porcine Embryos
Proceedings of the Society for Experimental Biology and Medicine, Vol.194(2), pp.144-148
06/1990
Handle:
https://hdl.handle.net/2376/116877
PMID: 2112254
Abstract
Abstract
Knowledge regarding the timing of embryonic expression of the mammalian genome is of relevance for the development of preimplantation diagnostic methods for human genetic diseases. For development of preimplantation diagnosis of lysosomal storage diseases, it will be necessary to know at which embryonic stage the genes for lysosomal enzymes are expressed. In previous studies by other investigators, it has been shown that lysosomal α- and β-galactosidase and β-glucuronidase in murine embryos increase 50- to 100-fold in activity between the two-cell and late blastocyst stage. We describe here expression of lysosomal β-galactosidase in preimplantation ovine (two-cell through midblastocyst) and porcine (two-cell through late blastocyst) embryos. Expression of β-galactosidase in ovine and porcine preimplantation embryos followed a similar rate of increase as that described for murine embryos. Activity of β-galactosidase increased over 10-fold between the two- to four-cell and midblastocyst stages in ovine embryos, and 300-fold between the two-to four-cell and late blastocyst stages in porcine embryos. Activity expressed on a per cell basis was relatively constant in ovine embryos, as has been described in murine embryos, and increased approximately 5-fold on a per cell basis in porcine embryos. Activity of β-galactosidase in ovine and porcine embryos initially was > 12-fold on a per cell or per embryo basis than in murine embryos evaluated. The knowledge of β-galactosidase embryonic expression may provide the basis for preimplantation diagnosis of genetic β-galactosidase deficiency in these species.
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Details
- Title
- Expression of β-Galactosidase in Preimplantation Ovine and Porcine Embryos
- Creators
- Robert D MurnaneRaymond W WrightKriss A HoffmanDavid J Prieur
- Publication Details
- Proceedings of the Society for Experimental Biology and Medicine, Vol.194(2), pp.144-148
- Academic Unit
- Animal Sciences, Department of
- Publisher
- SAGE Publications; London, England
- Identifiers
- 99900548188401842
- Language
- English
- Resource Type
- Journal article