Expression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli

Malgorzata Krause-Gruszczynska; Lieke B van Alphen; Omar A Oyarzabal; Thomas Alter; Ingrid Hänel; Annette Schliephake; Wolfgang König; Jos P M van Putten; Michael E Konkel; Steffen Backert

doi:10.1111/j.1574-6968.2007.00802.x

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Expression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli

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Expression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli

Malgorzata Krause-Gruszczynska, Lieke B van Alphen, Omar A Oyarzabal, Thomas Alter, Ingrid Hänel, Annette Schliephake, Wolfgang König, Jos P M van Putten, Michael E Konkel and Steffen Backert

FEMS microbiology letters, Vol.274(1), pp.9-16

09/2007

DOI: https://doi.org/10.1111/j.1574-6968.2007.00802.x

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https://hdl.handle.net/2376/107862

PMID: 17573935

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https://doi.org/10.1111/j.1574-6968.2007.00802.xView

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Abstract

Carrier Proteins - physiology

Immunohistochemistry

Amino Acid Sequence

Cell Line

Campylobacter jejuni - pathogenicity

Humans

Bacterial Outer Membrane Proteins - chemistry

Molecular Sequence Data

Campylobacter coli - genetics

Carrier Proteins - genetics

Sequence Analysis, Protein

Sequence Alignment

Campylobacter coli - metabolism

Campylobacter coli - pathogenicity

Polymerase Chain Reaction

Campylobacter jejuni - metabolism

Carrier Proteins - chemistry

Bacterial Outer Membrane Proteins - physiology

Mutation

Bacterial Outer Membrane Proteins - genetics

Campylobacter jejuni - genetics

Binding of Campylobacter jejuni and Campylobacter coli to host fibronectin is mediated by the 37 kDa outer membrane protein CadF. Immunoblot analysis of 58 C. jejuni and C. coli isolates of human and animal origin showed that CadF is expressed in every strain. In most C. jejuni isolates, a 37 kDa band (p37) and a less-prominent 32 kDa band (p32) reacted with the antibodies. In C. coli isolates, CadF was consistently larger with sizes of 39 kDa (p39) and 34 kDa (p34), respectively. PCR analysis and sequencing revealed the presence of a 39-bp insertion sequence in the cadF gene of C. coli strains, explaining the increased molecular size. Infection assays revealed that C. jejuni bound and invaded INT-407 epithelial cells much more efficiently than C. coli and that this difference was considerably reduced in isogenic cadF mutants. These results demonstrate that CadF is an important pathogenicity factor. The difference between CadF of C. jejuni and C. coli may potentially be exploited to discriminate these species in food and clinical specimens.

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Title: Expression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli
Creators: Malgorzata Krause-Gruszczynska - Department of Medical Microbiology, Otto von Guericke University Magdeburg, Magdeburg, Germany
Lieke B van Alphen
Omar A Oyarzabal
Thomas Alter
Ingrid Hänel
Annette Schliephake
Wolfgang König
Jos P M van Putten
Michael E Konkel
Steffen Backert
Publication Details: FEMS microbiology letters, Vol.274(1), pp.9-16
Academic Unit: Molecular Biosciences, School of
Publisher: England
Identifiers: 99900547105901842
Language: English
Resource Type: Journal article