Journal article
Extracellular ATP Acts on Jasmonate Signaling to Reinforce Plant Defense
Plant physiology (Bethesda), Vol.176(1), pp.511-523
01/2018
Handle:
https://hdl.handle.net/2376/110220
PMID: 29180381
Abstract
Damaged cells send various signals to stimulate defense responses. Recent identification and genetic studies of the plant purinoceptor, P2K1 (also known as DORN1), have demonstrated that extracellular ATP is a signal involved in plant stress responses, including wounding, perhaps to evoke plant defense. However, it remains largely unknown how extracellular ATP induces plant defense responses. Here, we demonstrate that extracellular ATP induces plant defense mediated through activation of the intracellular signaling of jasmonate (JA), a well-characterized defense hormone. In Arabidopsis (
) leaves, ATP pretreatment induced resistance against the necrotrophic fungus,
The induced resistance was enhanced in the P2K1 receptor overexpression line, but reduced in the receptor mutant,
-
Mining the transcriptome data revealed that ATP induces a set of JA-induced genes. In addition, the P2K1-associated coexpression network contains defense-related genes, including those encoding jasmonate ZIM-domain (JAZ) proteins, which play key roles as repressors of JA signaling. We examined whether extracellular ATP impacts the stability of JAZ1 in Arabidopsis. The results showed that the JAZ1 stability decreased in response to ATP addition in a proteasome-dependent manner. This reduction required intracellular signaling via second messengers-cytosolic calcium, reactive oxygen species, and nitric oxide. Interestingly, the ATP-induced JAZ1 degradation was attenuated in the JA receptor mutant,
, but not in the JA biosynthesis mutant,
, or upon addition of JA biosynthesis inhibitors. Immunoprecipitation analysis demonstrated that ATP increases the interaction between COI1 and JAZ1, suggesting direct cross talk between extracellular ATP and JA in intracellular signaling events. Taken together, these results suggest that extracellular ATP signaling directly impacts the JA signaling pathway to maximize plant defense responses.
Metrics
Details
- Title
- Extracellular ATP Acts on Jasmonate Signaling to Reinforce Plant Defense
- Creators
- Diwaker Tripathi - Department of Plant Pathology, Washington State University, Pullman, Washington 99164Tong Zhang - Divisions of Plant Sciences and Biochemistry, University of Missouri, Columbia, Missouri 65211Abraham J Koo - Divisions of Plant Sciences and Biochemistry, University of Missouri, Columbia, Missouri 65211Gary Stacey - Divisions of Plant Sciences and Biochemistry, University of Missouri, Columbia, Missouri 65211Kiwamu Tanaka - Department of Plant Pathology, Washington State University, Pullman, Washington 99164 kiwamu.tanaka@wsu.edu
- Publication Details
- Plant physiology (Bethesda), Vol.176(1), pp.511-523
- Academic Unit
- Plant Pathology, Department of
- Publisher
- United States
- Grant note
- R01 GM121445 / NIGMS NIH HHS R01GM121445 / NIH HHS
- Identifiers
- 99900546908801842
- Language
- English
- Resource Type
- Journal article