Journal article
Extracellular Alkalinization as a Defense Response in Potato Cells
Frontiers in plant science, Vol.8, pp.32-32
01/24/2017
Handle:
https://hdl.handle.net/2376/105085
PMCID: PMC5258701
PMID: 28174578
Abstract
A quantitative and robust bioassay to assess plant defense response is important for studies of disease resistance and also for the early identification of disease during pre- or non-symptomatic phases. An increase in extracellular pH is known to be an early defense response in plants. In this study, we demonstrate extracellular alkalinization as a defense response in potatoes. Using potato suspension cell cultures, we observed an alkalinization response against various pathogen- and plant-derived elicitors in a dose- and time-dependent manner. We also assessed the defense response against a variety of potato pathogens, such as protists (
Phytophthora infestans
and
Spongospora subterranea
) and fungi (
Verticillium dahliae
and
Colletotrichum coccodes
). Our results show that extracellular pH increases within 30 min in proportion to the number of pathogen spores added. Consistently with the alkalinization effect, the higher transcription level of several defense-related genes and production of reactive oxygen species was observed. Our results demonstrate that the alkalinization response is an effective marker to study early stages of defense response in potatoes.
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Details
- Title
- Extracellular Alkalinization as a Defense Response in Potato Cells
- Creators
- Natalia Moroz - Department of Plant Pathology, Washington State University, PullmanKaren R Fritch - Agricultural and Food Systems, Washington State University, PullmanMatthew J Marcec - Department of Plant Pathology, Washington State University, PullmanDiwaker Tripathi - Department of Plant Pathology, Washington State University, PullmanAndrei Smertenko - Molecular Plant Sciences Program, Washington State University, PullmanKiwamu Tanaka - Department of Plant Pathology, Washington State University, Pullman
- Publication Details
- Frontiers in plant science, Vol.8, pp.32-32
- Academic Unit
- Plant Pathology, Department of; Biological Chemistry, Institute of
- Publisher
- Frontiers Media S.A
- Identifiers
- 99900546956501842
- Language
- English
- Resource Type
- Journal article