Journal article
Extraction of sleep-promoting factor S from cerebrospinal fluid and from brains of sleep-deprived animals
Journal of neurophysiology, Vol.38(6), pp.1299-1311
11/1975
Handle:
https://hdl.handle.net/2376/116239
PMID: 1221075
Abstract
Sleep-promoting factor (factor S) was extracted, partially purified, and concentrated from cerebrospinal fluid and from acid-acetone extracts of brain stem anc cortex of sleep-deprived goats and sheep. 2. Solutes greater than 500 daltons were largely removed by serial ultrafiltrations through molecular sieves (Amicon membranes UM10 and UM05); solutes less than 350 daltons were largely eliminated by gel filtration through Sephadex G10 columns. Sleep-promoting activity was found in a fraction eluted prior to [14C] sucrose marker. 3. Concentrated fraction were infused intraventricularly in rats (0.1 ml in 30 min just prior to 12-h dark cycle) and in rabbits (0.3 ml in 90 min in morning). Sleep-promoting activity was assayed by decrease in nocturnal locomotor activity of rats and by duration and amplitude of slow-wave cortical EEG in rabbits.
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Details
- Title
- Extraction of sleep-promoting factor S from cerebrospinal fluid and from brains of sleep-deprived animals
- Creators
- J R PappenheimerG KoskiV FenclM L KarnovskyJ Krueger
- Publication Details
- Journal of neurophysiology, Vol.38(6), pp.1299-1311
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- United States
- Identifiers
- 99900548161201842
- Language
- English
- Resource Type
- Journal article