Journal article
Failure to detect Trichinella spiralis p43 in isolated host nuclei and in irradiated larvae of infected muscle cells which express the infected cell phenotype
Molecular and biochemical parasitology, Vol.67(2), pp.225-234
1994
Handle:
https://hdl.handle.net/2376/114752
PMID: 7870127
Abstract
Infection by
Trichinella spiralis induces host muscle cells to become repositioned within the cell cycle and to lose differentiated skeletal muscle characteristics. Antibodies to a 43-kDa excretory-secretory (ES) protein (p43) also bind to infected host cell nuclei. Neither the identity of these nuclear antigens nor their role in inducing the infected cell phenotype is known. To address these issues, infected cell nuclei were isolated and nuclear antigens analyzed with several antibody preparations to p43. Four antibody preparations to p43 recognized 43-, 45-, 50-, 67- and 71-kDa proteins in ES extracts. The prominent proteins recognized by these antibodies in host nuclear antigen extracts were 71, 79, 86 and 97 kDa. Less prominent proteins of approximately 43 and 45 kDa were detected in nuclear extracts. However, antibodies which specifically recognized 1043 failed to bind detectably with in situ and isolated host nuclei and nuclear extracts. Expression of p43 was analyzed in host cells infected by newborn larvae irradiated with
60Co. This treatment prevented expression of detectable levels of p43 in resulting muscle larvae, while infected muscle cells displayed typical infected cell characteristics. However, anti-p43 antibodies which recognized multiple ES and nuclear proteins did stain nuclei of irradiated larva-infected cells, albeit at reduced levels. The results raise doubts that p43 is required for induction of the infected cell phenotype. Nevertheless, nuclear antigens recognized by anti-p43 antibodies remain as candidates for influencing this phenotype.
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Details
- Title
- Failure to detect Trichinella spiralis p43 in isolated host nuclei and in irradiated larvae of infected muscle cells which express the infected cell phenotype
- Creators
- Douglas P Jasmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040 USASufang Yao - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040 USADemetris Vassilatis - Department of Genetics and Molecular Biology, Merck Research Laboratories, Rahway, NJ USADickson Despommier - Division of Environmental Sciences and Department of Microbiology, Columbia University, New York, NY USASusan M Neary - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040 USA
- Publication Details
- Molecular and biochemical parasitology, Vol.67(2), pp.225-234
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547530101842
- Language
- English
- Resource Type
- Journal article