Journal article
Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam-exploded softwood substrates
Biotechnology and bioengineering, Vol.77(6), pp.678-684
03/20/2002
Handle:
https://hdl.handle.net/2376/109022
PMID: 11807763
Abstract
The enzymatic digestibility of steam-exploded Douglas-fir wood chips (steam exploded at 195 degrees C, 4.5 min, and 4.5% (w/w) SO(2)) was significantly improved using an optimized alkaline peroxide treatment. Best hydrolysis yields were attained when the steam-exploded material was post-treated with 1% hydrogen peroxide at pH 11.5 and 80 degrees C for 45 min. This alkaline peroxide treatment was applied directly to the water-washed, steam-exploded material eliminating the need for independent alkali treatment with 0.4% NaOH, which has been traditionally used to post-treat wood samples to try to remove residual lignin. Approximately 90% of the lignin in the original wood was solubilized by this novel procedure, leaving a cellulose-rich residue that was completely hydrolyzed within 48 h, using an enzyme loading of 10 FPU/g cellulose. About 82% of the originally available polysaccharide components of the wood could be recovered. The 18% of the carbohydrate that was not recovered was lost primarily to sugar degradation during steam explosion.
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Details
- Title
- Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam-exploded softwood substrates
- Creators
- Bin Yang - Faculty of Forestry, Forest Products Biotechnology, University of British Columbia, Vancouver, CanadaAbdel BoussaidShawn D MansfieldDavid J GreggJohn N Saddler
- Publication Details
- Biotechnology and bioengineering, Vol.77(6), pp.678-684
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- United States
- Identifiers
- 99900547514101842
- Language
- English
- Resource Type
- Journal article