Journal article
Fe-N-C Single-Atom Nanozymes for the Intracellular Hydrogen Peroxide Detection
Analytical chemistry (Washington), Vol.91(18), pp.11994-11999
09/17/2019
PMID: 31436084
Abstract
Recently, in situ detection of hydrogen peroxide (HO) generated from live cells have caused tremendous attention, because it is of great significance in the control of multiple biological processes. Herein, Fe-N-C single-atom nanozymes (Fe-N-C SAzymes) with intrinsic peroxidase-like activity were successfully prepared via high-temperature calcination using FeCl , glucose, and dicyandiamide as precursors. The Fe-N-C SAzymes with FeN as active sites were similar to natural metalloproteases, which can specifically enhance the peroxidase-like activity rather than oxidase-like activity. Accordingly, owing to the excellent catalytic efficiency of the Fe-N-C SAzymes, colorimetric biosensing of H O in vitro was performed via a typical 3,3',5,5'-tetramethylbenzidine induced an allochroic reaction, demonstrating the satisfactory specificity and sensitivity. With regard to the practical application, in situ detection of H O generated from the Hela cells by the Fe-N-C SAzymes was also performed, which can expand the applications of the newborn SAzymes.
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Details
- Title
- Fe-N-C Single-Atom Nanozymes for the Intracellular Hydrogen Peroxide Detection
- Creators
- Lei Jiao - Central China Normal UniversityWeiqing Xu - Central China Normal UniversityHongye Yan - Central China Normal UniversityYu Wu - Central China Normal UniversityChunrong Liu - Central China Normal UniversityDan Du - Washington State UniversityYuehe Lin - Washington State UniversityChengzhou Zhu - Central China Normal University
- Publication Details
- Analytical chemistry (Washington), Vol.91(18), pp.11994-11999
- Academic Unit
- School of Mechanical and Materials Engineering
- Identifiers
- 99901227645601842
- Language
- English
- Resource Type
- Journal article