Fe(III) Reduction and U(VI) Immobilization by Paenibacillus sp. Strain 300A, Isolated from Hanford 300A Subsurface Sediments

Bulbul Ahmed; Bin Cao; Jeffrey S McLean; Tuba Ica; Alice Dohnalkova; Ozlem Istanbullu; Akin Paksoy; Jim K Fredrickson; Haluk Beyenal

doi:10.1128/AEM.01844-12

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Fe(III) Reduction and U(VI) Immobilization by Paenibacillus sp. Strain 300A, Isolated from Hanford 300A Subsurface Sediments

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Fe(III) Reduction and U(VI) Immobilization by Paenibacillus sp. Strain 300A, Isolated from Hanford 300A Subsurface Sediments

Bulbul Ahmed, Bin Cao, Jeffrey S McLean, Tuba Ica, Alice Dohnalkova, Ozlem Istanbullu, Akin Paksoy, Jim K Fredrickson and Haluk Beyenal

Applied and environmental microbiology, Vol.78(22), pp.8001-8009

11/2012

DOI: https://doi.org/10.1128/AEM.01844-12

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https://hdl.handle.net/2376/115775

PMCID: PMC3485948

PMID: 22961903

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Abstract

Geomicrobiology

A facultative iron-reducing [Fe(III)-reducing] Paenibacillus sp. strain was isolated from Hanford 300A subsurface sediment biofilms that was capable of reducing soluble Fe(III) complexes [Fe(III)-nitrilotriacetic acid and Fe(III)-citrate] but unable to reduce poorly crystalline ferrihydrite (Fh). However, Paenibacillus sp. 300A was capable of reducing Fh in the presence of low concentrations (2 μM) of either of the electron transfer mediators (ETMs) flavin mononucleotide (FMN) or anthraquinone-2,6-disulfonate (AQDS). Maximum initial Fh reduction rates were observed at catalytic concentrations (<10 μM) of either FMN or AQDS. Higher FMN concentrations inhibited Fh reduction, while increased AQDS concentrations did not. We also found that Paenibacillus sp. 300A could reduce Fh in the presence of natural ETMs from Hanford 300A subsurface sediments. In the absence of ETMs, Paenibacillus sp. 300A was capable of immobilizing U(VI) through both reduction and adsorption. The relative contributions of adsorption and microbial reduction to U(VI) removal from the aqueous phase were ∼7:3 in PIPES [piperazine- N , N ′ - bis(2-ethanesulfonic acid)] and ∼1:4 in bicarbonate buffer. Our study demonstrated that Paenibacillus sp. 300A catalyzes Fe(III) reduction and U(VI) immobilization and that these reactions benefit from externally added or naturally existing ETMs in 300A subsurface sediments.

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Title: Fe(III) Reduction and U(VI) Immobilization by Paenibacillus sp. Strain 300A, Isolated from Hanford 300A Subsurface Sediments
Creators: Bulbul Ahmed - The Gene and Linda Voiland School of Chemical Engineering, Washington State University, Pullman, Washington, USA
Bin Cao - The Gene and Linda Voiland School of Chemical Engineering, Washington State University, Pullman, Washington, USA
Jeffrey S McLean - J. Craig Venter Institute, San Diego, California, USA
Tuba Ica - The Gene and Linda Voiland School of Chemical Engineering, Washington State University, Pullman, Washington, USA
Alice Dohnalkova - Pacific Northwest National Laboratory, Richland, Washington, USA
Ozlem Istanbullu - The Gene and Linda Voiland School of Chemical Engineering, Washington State University, Pullman, Washington, USA
Akin Paksoy - The Gene and Linda Voiland School of Chemical Engineering, Washington State University, Pullman, Washington, USA
Jim K Fredrickson - Pacific Northwest National Laboratory, Richland, Washington, USA
Haluk Beyenal - The Gene and Linda Voiland School of Chemical Engineering, Washington State University, Pullman, Washington, USA
Publication Details: Applied and environmental microbiology, Vol.78(22), pp.8001-8009
Academic Unit: Chemical Engineering and Bioengineering, School of
Publisher: American Society for Microbiology; 1752 N St., N.W., Washington, DC
Identifiers: 99900548063601842
Language: English
Resource Type: Journal article