Journal article
Fecal Microbial Community Structure Is Stable over Time and Related to Variation in Macronutrient and Micronutrient Intakes in Lactating Women
The Journal of nutrition, Vol.145(10), pp.2379-2388
10/2015
Handle:
https://hdl.handle.net/2376/118216
PMID: 26311809
Abstract
The fecal microbiota has been characterized in some adult populations, but little is known about its community structure during lactation.
We characterized the maternal fecal microbiome during lactation and explored possible mediating factors such as nutrition.
Fecal samples were collected from 20 lactating women from 2 d to 6 mo postpartum, and bacterial taxa were characterized with the use of high-throughput sequencing. Bacterial community structure (at each taxonomic level) and relations between bacterial taxa and environmental and dietary variables were visualized and analyzed with the use of stacked bar charts, principal component analysis, and multivariate analyses such as nonmetric multidimensional scaling and canonical correlation analysis.
Complex bacterial community structure was somewhat similar to those previously published for other adult populations (although there were some notable differences), and there were no clear associations with time postpartum or anthropometric or environmental variables. However, Spearman rank correlations suggested that increased intake of pantothenic acid, riboflavin, vitamin B-6, and vitamin B-12 were related to increased relative abundance of Prevotella (r = 0.45, 0.39, 0.34, and 0.24, respectively; P ≤ 0.01) and decreased relative abundance of Bacteroides (r = -0.55, -0.46, -0.32, and -0.35, respectively; P ≤ 0.01). Intakes of copper, magnesium, manganese, and molybdenum were positively associated with Firmicutes (r = 0.33, 0.38, 0.44, and 0.51, respectively; P ≤ 0.01) and negatively associated with Bacteroidetes (r = -0.38, -0.44, -0.48, and -0.53, respectively; P ≤ 0.01). Overall, data consistently suggest that increased consumption of a more nutrient- and calorie-rich diet was positively associated with relative abundance of Firmicutes.
The fecal microbiome of lactating women is relatively stable in the postpartum period and somewhat similar to that of other adult populations. Variation in dietary constituents may be related to that of relative abundance of individual bacterial taxa. Controlled dietary intervention studies will be required to determine whether these associations are causal in nature.
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Details
- Title
- Fecal Microbial Community Structure Is Stable over Time and Related to Variation in Macronutrient and Micronutrient Intakes in Lactating Women
- Creators
- Janae M Carrothers - School of Biological Sciences, Washington State University, Pullman, WA; andMara A York - School of Biological Sciences, Washington State University, Pullman, WA; andSarah L Brooker - Department of Animal and Veterinary Science, Program in Bioinformatics and Computational BiologyKimberly A Lackey - School of Biological Sciences, Washington State University, Pullman, WA; andJanet E Williams - Department of Animal and Veterinary Science, Program in Bioinformatics and Computational BiologyBahman Shafii - Statistical Programs, College of Agricultural and Life Sciences, andWilliam J Price - Statistical Programs, College of Agricultural and Life Sciences, andMatthew L Settles - Institute for Bioinformatics and Evolutionary Studies, Department of Biological Sciences, University of Idaho, Moscow, IDMark A McGuire - Department of Animal and Veterinary Science, Program in Bioinformatics and Computational BiologyMichelle K McGuire - School of Biological Sciences, Washington State University, Pullman, WA; and smcguire@wsu.edu
- Publication Details
- The Journal of nutrition, Vol.145(10), pp.2379-2388
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- United States
- Grant note
- P30 GM103324 / NIGMS NIH HHS
- Identifiers
- 99900548104601842
- Language
- English
- Resource Type
- Journal article