Journal article
Feeding cells induced by phytoparasitic nematodes require gamma-tubulin ring complex for microtubule reorganization
PLoS pathogens, Vol.7(12), pp.e1002343-e1002343
2011
Handle:
https://hdl.handle.net/2376/113732
PMCID: PMC3228788
PMID: 22144887
Abstract
Reorganization of the microtubule network is important for the fast isodiametric expansion of giant-feeding cells induced by root-knot nematodes. The efficiency of microtubule reorganization depends on the nucleation of new microtubules, their elongation rate and activity of microtubule severing factors. New microtubules in plants are nucleated by cytoplasmic or microtubule-bound c-tubulin ring complexes. Here we investigate the requirement of c-tubulin complexes for giant feeding cells development using the interaction between Arabidopsis and Meloidogyne spp. as a model system. Immunocytochemical analyses demonstrate that c-tubulin localizes to both cortical cytoplasm and mitotic microtubule arrays of the giant cells where it can associate with microtubules. The transcripts of two Arabidopsis c-tubulin (TUBG1 and TUBG2) and two c-tubulin complex proteins genes (GCP3 and GCP4) are upregulated in galls. Electron microscopy demonstrates association of GCP3 and c-tubulin as part of a complex in the cytoplasm of giant cells. Knockout of either or both c-tubulin genes results in the gene dose-dependent alteration of the morphology of feeding site and failure of nematode life cycle completion. We conclude that the c-tubulin complex is essential for the control of microtubular network remodelling in the course of initiation and development of giant-feeding cells, and for the successful reproduction of nematodes in their plant hosts.
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Details
- Title
- Feeding cells induced by phytoparasitic nematodes require gamma-tubulin ring complex for microtubule reorganization
- Creators
- Mohamed Youssef Banora - Interactions Biotiques et Santé VégétaleNatalia Rodiuc - Interactions Biotiques et Santé VégétaleFabien Baldacci - Interactions Biotiques et Santé VégétaleAndrei Smertenko - School of Biological and Biomedical SciencesTeresa Bleve-Zacheo - Consiglio Nazionale delle RicercheMaria Teresa Mellilo - Consiglio Nazionale delle RicercheMansour Karimi - Department of plant systems biologyPierre Hilson - Department of plant systems biologyJean-Luc Evrard - Institut de biologie moléculaire des plantesBruno Favery - Interactions Biotiques et Santé VégétaleGilbert Engler - Interactions Biotiques et Santé VégétalePierre Abad - Interactions Biotiques et Santé VégétaleJanice De Almeida-Engler - Interactions Biotiques et Santé Végétale
- Publication Details
- PLoS pathogens, Vol.7(12), pp.e1002343-e1002343
- Academic Unit
- Biological Chemistry, Institute of
- Publisher
- Public Library of Science
- Identifiers
- 99900548347001842
- Language
- English
- Resource Type
- Journal article