Journal article
Fermentation of levoglucosan with oleaginous yeasts for lipid production
Bioresource technology, Vol.133, pp.183-189
04/2013
Handle:
https://hdl.handle.net/2376/105323
PMID: 23425586
Abstract
[Display omitted]
► A new process of directly conversion of levoglucosan to lipid was proposed. ► Oleaginous yeasts Rhodotorula glutinis and Rhodosporidium toruloides showed best growth in LG. ► Enzyme activities of LG kinase from R. glutinis and R. toruloides were determined. ► LG aqueous phase by three detoxification methods was fermented by R. glutinis.
This paper reports the production of lipids from non-hydrolyzed levoglucosan (LG) by oleaginous yeasts Rhodosporidium toruloides and Rhodotorula glutinis. Enzyme activity tests of LG kinases from both yeasts indicated that the phosphorylation pathway of LG to glucose-6-phosphate existed. The highest enzyme activity obtained for R. glutinis was 0.22U/mg of protein. The highest cell mass and lipid production by R. glutinis were 6.8 and 2.7g/L, respectively from pure LG, and 3.3 and 0.78g/L from a pyrolytic LG aqueous phase detoxified by ethyl acetate extraction, rotary evaporation and activated carbon. This corresponded to a lipid yield of 13.5wt.% for pure LG and only 3.9wt.% for LG in pyrolysis oil.
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Details
- Title
- Fermentation of levoglucosan with oleaginous yeasts for lipid production
- Creators
- Jieni LianManuel Garcia-PerezShulin Chen
- Publication Details
- Bioresource technology, Vol.133, pp.183-189
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900546698801842
- Language
- English
- Resource Type
- Journal article