Fertilization in vitro increases non-disjunction during early cleavage divisions in a mouse model system

Christopher J Bean; Terry J Hassold; LuAnn Judis; Patricia A Hunt

doi:10.1093/humrep/17.9.2362

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Fertilization in vitro increases non-disjunction during early cleavage divisions in a mouse model system

Journal article

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Fertilization in vitro increases non-disjunction during early cleavage divisions in a mouse model system

Christopher J Bean, Terry J Hassold, LuAnn Judis and Patricia A Hunt

Human reproduction (Oxford), Vol.17(9), pp.2362-2367

09/2002

DOI: https://doi.org/10.1093/humrep/17.9.2362

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https://hdl.handle.net/2376/116914

PMID: 12202426

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Abstract

Cleavage Stage, Ovum - physiology

Aneuploidy

Male

Embryonic and Fetal Development

Oxygen - metabolism

Y Chromosome - genetics

Embryo, Mammalian - physiology

Animals

Fertilization in Vitro

Chromosome Segregation - physiology

Mosaicism

Mice

Mice, Inbred BALB C

Nondisjunction, Genetic

We have been studying an unusual mouse-the BALB/cWt (Wt) male-in which the Y chromosome is susceptible to high rates of mitotic non-disjunction, particularly at the first two cleavage divisions. As these are the same divisions that human embryos generated through assisted reproductive technology must complete in an artificial setting, analysis of the Wt Y chromosome allows us to examine the effect of fertilization and culture in vitro on mammalian chromosome segregation. We performed standard mouse IVF, cultured embryos in 5% CO2 in air or in a lowered oxygen atmosphere, and used fluorescence in-situ hybridization to examine the sex chromosome constitutions of 2-, 4-, 8- and 16-cell stage Wt Y-bearing embryos. We observed a significant increase in mosaic sex chromosome aneuploidy at each embryonic stage in embryos cultured in 5% CO2 in air, but under lowered oxygen conditions mosaicism returned to control (in-vivo) levels. Our results demonstrate that slight alterations in in-vitro conditions may have a considerable impact on the genetic quality of assisted reproductive technology-derived embryos and suggest that the genetic quality of embryos should be a fundamental concern in the development of new culture systems for clinical use.

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Title: Fertilization in vitro increases non-disjunction during early cleavage divisions in a mouse model system
Creators: Christopher J Bean - Department of Genetics and the Center for Human Genetics, Case Western Reserve University and University Hospitals of Cleveland, Cleveland, OH 44106, USA
Terry J Hassold
LuAnn Judis
Patricia A Hunt
Publication Details: Human reproduction (Oxford), Vol.17(9), pp.2362-2367
Academic Unit: Molecular Biosciences, School of
Publisher: England
Grant note: GM08056 / NIGMS NIH HHS HD31866 / NICHD NIH HHS HD21341 / NICHD NIH HHS
Identifiers: 99900547768801842
Language: English
Resource Type: Journal article