Journal article
Fibronectin-Facilitated Invasion of T84 Eukaryotic Cells by Campylobacter jejuni Occurs Preferentially at the Basolateral Cell Surface
Infection and immunity, Vol.70(12), pp.6665-6671
12/2002
Handle:
https://hdl.handle.net/2376/102078
PMCID: PMC133038
PMID: 12438340
Abstract
Previous studies have indicated that the ability to bind to fibronectin is a key feature in successful cell invasion by
Campylobacter jejuni
. Given the spatial distribution of fibronectin and the architecture of the epithelium, this suggests the possibility that
C. jejuni
cell invasion might preferentially occur at the basolateral cell surface. To test this hypothesis, we examined the interaction of
C. jejuni
with T84 human colonic cells. When grown under the appropriate conditions, T84 cells form a polarized cell monolayer.
C. jejuni
translocation of a T84 cell monolayer appeared to occur via a paracellular (extracellular) route as opposed to a transcellular (intracellular) route based on the finding that a
C. jejuni
noninvasive mutant translocated as efficiently as its isogenic parent. Additional studies revealed that two distinct
C. jejuni
wild-type isolates could compete with one another for host cell receptors, whereas a
C. jejuni
fibronectin-binding-deficient mutant could not compete with a wild-type isolate for host cell receptors. Further,
C. jejuni
adherence and internalization were significantly inhibited by antifibronectin antibodies but only when cells were first treated with EGTA to expose basolateral cell surfaces. Together, these results support the theory that
C. jejuni
invasion occurs preferentially at the basolateral surface of eukaryotic cells.
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Details
- Title
- Fibronectin-Facilitated Invasion of T84 Eukaryotic Cells by Campylobacter jejuni Occurs Preferentially at the Basolateral Cell Surface
- Creators
- Marshall R Monteville - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164Michael E Konkel - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164
- Publication Details
- Infection and immunity, Vol.70(12), pp.6665-6671
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546671501842
- Language
- English
- Resource Type
- Journal article