Journal article
Flow cytometric assays to detect platelet activation and aggregation in device‐implanted calves
Journal of biomedical materials research, Vol.41(2), pp.312-321
08/1998
Handle:
https://hdl.handle.net/2376/108342
PMID: 9638537
Abstract
Cardiovascular device development often relies upon large‐animal models to assess blood biocompatibility prior to initiating clinical trials. Unfortunately, the amount of information gleaned from such trials is limited by simple assays that do not take full advantage of immuno‐technological advances that increasingly are applied in clinical studies. Thus we have developed and tested new flow cytometric techniques for measuring circulating activated bovine platelets and platelet microaggregates. Monoclonal antibodies (MAbs) raised against both activated and quiescent bovine platelets were incubated with control and PMA‐ or ADP‐stimulated whole blood. Selected MAbs detected activated bovine platelets and platelet microaggregates in vitro with flow cytometry. Five calves implanted with one of two designs of nonpulsatile ventricular‐assist devices (VADs) were followed with these assays prior to and during VAD implantation. Circulating activated bovine platelets and microaggregates increased after implantation in all animals and, alternatively, remained elevated or returned toward preimplant levels. Platelet activation percentages as detected temporally by three MAbs were correlated with one another, and platelet activation was correlated with microaggregate formation. In summary, these new methods for the sensitive measurement of circulating activated bovine platelets and microaggregates may provide valuable information for the development and assessment of future cardiovascular device designs. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 41, 312–321, 1998.
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Details
- Title
- Flow cytometric assays to detect platelet activation and aggregation in device‐implanted calves
- Creators
- Linda C BakerWilliam C DavisJacqueline AutieriMary J WatachKenji YamazakiPhilip LitwakWilliam R Wagner
- Publication Details
- Journal of biomedical materials research, Vol.41(2), pp.312-321
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- John Wiley & Sons, Inc; New York
- Number of pages
- 10
- Grant note
- McGowan Center for Artificial Organs National Institutes of Health (NO1‐HV‐58155)
- Identifiers
- 99900547695001842
- Language
- English
- Resource Type
- Journal article