Journal article
Flow cytometric detection of host cell apoptosis induced by bacterial infection
Methods in cell science, Vol.22(2), pp.209-215
06/2000
Handle:
https://hdl.handle.net/2376/113669
PMID: 11264955
Abstract
We detail two methods for detection of cell death induced by infection of a human monocytic cell line with invasive Campylobacter bacteria. Staining with a natural ligand for exposed phosphatidylserine residues coupled with propidum iodide discriminated between apoptosis and necrosis. Additionally, cells infected with a bacterial strain expressing green fluorescent protein stained with dye sensitive to mitochondrial membrane potential demonstrated a direct association of bacteria with dying cells. Analyses of cells stained by these methods employing flow cytometry enumerated proportions of cell populations undergoing either apoptosis or necrosis after bacterial infection in vitro.
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Details
- Title
- Flow cytometric detection of host cell apoptosis induced by bacterial infection
- Creators
- Michael KonkelPhilip Mixter
- Publication Details
- Methods in cell science, Vol.22(2), pp.209-215
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Kluwer Academic Publishers; Dordrecht
- Identifiers
- 99900547701301842
- Language
- English
- Resource Type
- Journal article