Journal article
Fluorescent silicon nanoparticles-based ratiometric fluorescence immunoassay for sensitive detection of ethyl carbamate in red wine
Sensors and actuators. B, Chemical, Vol.255, pp.2742-2749
02/01/2018
Abstract
Herein, a ratiometric fluorescence (RF) enzyme-linked immunosorbent assay (RF-ELISA) for sensitive detection of ethyl carbamate (EC) was developed by introducing fluorescent silicon nanoparticles (Si NPs) into the chromogenic substrate system (o-phenylenediamine (OPD)/H2O2) of a conventional horseradish peroxidase (HRP)-based ELISA platform to assemble a RF-based signal output system. For this system, the fluorescence of Si NPs at 440 nm (I-440) was acted as the reference signal which could be efficiently quenched by 2,3-diaminophenazine (DAP), the HRP-catalyzed oxidation product of OPD; Meanwhile, the fluorescence of DAP at 570 nm (I-570) was served as response signal. Therefore, variation in the amount of HRP labeled secondary antibody bound on the microplate which is associated with antibody-antigen recognition events in conventional HRP-based ELISA could be transferred into a more sensitive RF signal (I-520/I-440). On the basis of monoclonal antibody (mAb) which could specifically recognize EC derivative, xanthyl ethyl carbamate (XEC), a Si NPs-based RF-ELISA for EC via a simple pre-analysis derivatization was developed. When detecting the EC content in red wine, this method exhibits a working range from 3.9 to 105.0 mu g/L and a limit of detection (LOD) of 2.6 mu g/L with excellent specificity, accuracy and reproducibility. The sensitivity is approximately 33-fold higher than that of traditional colorimetric ELISA. The proposed Si NPs-based RF-ELISA is not only highly suitable for screening EC in a large number of samples, but also provides a potential platform for high-throughput and sensitive determination of other analytes for food safety monitoring. (C) 2017 Elsevier B.V. All rights reserved.
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Details
- Title
- Fluorescent silicon nanoparticles-based ratiometric fluorescence immunoassay for sensitive detection of ethyl carbamate in red wine
- Creators
- Lin Luo - Washington State UniversityYang Song - Washington State UniversityChengzhou Zhu - Washington State UniversityShaofang Fu - Washington State UniversityQiurong Shi - Washington State UniversityYuan-Ming Sun - South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Guangdong, Peoples R ChinaBaozhu Jia - Guangdong Univ Educ, Coll Biol & Food Engn, Guangzhou 510303, Guangdong, Peoples R ChinaDan Du - Washington State UniversityZhen-Lin Xu - South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Guangdong, Peoples R ChinaYuehe Lin - Washington State University
- Publication Details
- Sensors and actuators. B, Chemical, Vol.255, pp.2742-2749
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- 21575047 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 20156020205002 / Science and Technology Foundation of Guangdong Province 2014A030306026 / Guangdong Natural Science Funds for Distinguished Young Scholar
- Identifiers
- 99901227639101842
- Language
- English
- Resource Type
- Journal article