Journal article
Fluorographic detection of radioactivity in polyacrylamide gels with 2,5-diphenyloxazole in acetic acid and its comparison with existing procedures
Biochemical journal, Vol.209(1), pp.281-284
01/01/1983
Handle:
https://hdl.handle.net/2376/112495
PMCID: PMC1154087
PMID: 6847617
Abstract
A fluorographic procedure was optimized which utilized acetic acid as the solvent for 2,5-diphenyloxazole (PPO). This procedure was then compared with existing fluorographic procedures which utilize PPO in dimethyl sulphoxide or sodium salicylate in water, and a commercially available fluorographic solution. En3Hance (New England Nuclear Corp.). A comparison of the four methods revealed that all of the procedures resulted in essentially the same efficiency of radioactivity detection. The acetic acid/PPO procedure was found to have several technical advantages. There is no need to pre-fix proteins in gels, and either agarose or acrylamide gels can be used. The acetic acid/PPO procedure was found to be a simple, sensitive and efficient alternative fluorographic method.
Metrics
Details
- Title
- Fluorographic detection of radioactivity in polyacrylamide gels with 2,5-diphenyloxazole in acetic acid and its comparison with existing procedures
- Creators
- M K SkinnerM D Griswold
- Publication Details
- Biochemical journal, Vol.209(1), pp.281-284
- Academic Unit
- Biological Sciences, School of
- Publisher
- England
- Grant note
- HD 00263 / NICHD NIH HHS HD 10808 / NICHD NIH HHS
- Identifiers
- 99900547623101842
- Language
- English
- Resource Type
- Journal article