Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15:K52:H1 clonal group from humans and dogs in Australia

Joanne L Platell; Rowland N Cobbold; James R Johnson; Connie R Clabots; Darren J Trott

doi:10.1016/j.cimid.2012.02.002

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Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15:K52:H1 clonal group from humans and dogs in Australia

Journal article

Peer reviewed

Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15:K52:H1 clonal group from humans and dogs in Australia

Joanne L Platell, Rowland N Cobbold, James R Johnson, Connie R Clabots and Darren J Trott

Comparative immunology, microbiology and infectious diseases, Vol.35(4), pp.319-324

07/2012

DOI: https://doi.org/10.1016/j.cimid.2012.02.002

Handle:

https://hdl.handle.net/2376/113462

PMID: 22385907

Abstract

Escherichia coli Infections - drug therapy

Genes, Bacterial

Escherichia coli - drug effects

Humans

Virulence

Dog Diseases - microbiology

Escherichia coli Infections - microbiology

Genotype

Phylogeny

Electrophoresis, Gel, Pulsed-Field

Animals

Dog Diseases - drug therapy

Escherichia coli - genetics

Escherichia coli - isolation & purification

Bacterial Typing Techniques

Fluoroquinolones - pharmacology

Dogs

Female

Anti-Bacterial Agents - pharmacology

Clone Cells

Escherichia coli Infections - veterinary

Polymorphism, Single Nucleotide

Australia

Drug Resistance, Bacterial - genetics

Antimicrobial-resistant extraintestinal pathogenic Escherichia coli (ExPEC) impact both human and veterinary medicine. One ExPEC clonal group that has become increasingly multidrug-resistant is serotype O15:K52:H1. Accordingly, we sought O15:K52:H1 strains among fluoroquinolone-resistant (FQ(r)) E. coli clinical isolates from humans (n=582) and dogs (n=120) in Australia. The phylogenetic group D isolates (267/702; 38%) were screened for O15:K52:H1-specific single-nucleotide polymorphisms (SNPs) in fumC and the O15 rfb variant. The 34 so-identified O15:K52:H1 isolates (33 human, 1 canine) underwent antimicrobial susceptibility profiling, virulence genotyping, and macrorestriction profiling. Although susceptibility profiles varied, the 34 isolates were closely related by pulsed-field gel electrophoresis and exhibited typical O15:K52:H1-associated virulence profiles (complete pap operon, F16 papA allele, papG allele II, iha, fimH, sat, fyuA, iutA, kpsMII, ompT). The canine isolate closely resembled human isolates. Thus, O15:K52:H1 strains contribute to the FQ(r) ExPEC population in Australia and may potentially be transferred between humans and dogs.

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Title: Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15:K52:H1 clonal group from humans and dogs in Australia
Creators: Joanne L Platell - School of Veterinary Science, The University of Queensland, Gatton, Queensland, Australia. j.platell@uq.edu.au
Rowland N Cobbold
James R Johnson
Connie R Clabots
Darren J Trott
Publication Details: Comparative immunology, microbiology and infectious diseases, Vol.35(4), pp.319-324
Publisher: England
Identifiers: 99900547317201842
Language: English
Resource Type: Journal article

Fluoroquinolone-resistant extraintestinal Escherichia coli clinical isolates representing the O15:K52:H1 clonal group from humans and dogs in Australia

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