Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre

Siyu Guo; Huub J M Brouwers; Rowland N Cobbold; Joanne L Platell; Toni A Chapman; Vanessa R Barrs; James R Johnson; Darren J Trott

doi:10.1093/jac/dks515

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Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre

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Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre

Siyu Guo, Huub J M Brouwers, Rowland N Cobbold, Joanne L Platell, Toni A Chapman, Vanessa R Barrs, James R Johnson and Darren J Trott

Journal of antimicrobial chemotherapy, Vol.68(5), pp.1025-1031

05/2013

DOI: https://doi.org/10.1093/jac/dks515

Handle:

https://hdl.handle.net/2376/114831

PMID: 23299573

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Abstract

Carrier State - epidemiology

Prevalence

Escherichia coli - drug effects

Carrier State - microbiology

Drug Resistance, Bacterial

Escherichia coli Infections - microbiology

Hospitals, Animal

Phylogeny

Australia - epidemiology

Molecular Typing

Escherichia coli Infections - epidemiology

Carrier State - veterinary

Animals

Molecular Epidemiology

Escherichia coli - genetics

Escherichia coli - isolation & purification

Fluoroquinolones - pharmacology

Dogs

Random Amplified Polymorphic DNA Technique

Anti-Bacterial Agents - pharmacology

Escherichia coli Infections - veterinary

Cluster Analysis

Escherichia coli - classification

To determine rates of carriage of fluoroquinolone-resistant Escherichia coli and extraintestinal pathogenic E. coli (ExPEC) among dogs in a specialist referral hospital and to examine the population structure of the isolates. Fluoroquinolone-resistant faecal E. coli isolates (n = 232, from 23 of 123 dogs) recovered from hospitalized dogs in a veterinary referral centre in Sydney, Australia, over 140 days in 2009 were characterized by phylogenetic grouping, virulence genotyping and random amplified polymorphic DNA (RAPD) analysis. The RAPD dendrogram for representative isolates showed one group B2-associated cluster and three group D-associated clusters; each contained isolates with closely related ExPEC-associated virulence profiles. All group B2 faecal isolates represented the O25b-ST131 clonal group and were closely related to recent canine extraintestinal ST131 clinical isolates from the east coast of Australia by RAPD analysis. Hospitalized dogs may carry fluoroquinolone-resistant ExPEC in their faeces, including those representing O25b-ST131.

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Title: Fluoroquinolone-resistant extraintestinal pathogenic Escherichia coli, including O25b-ST131, isolated from faeces of hospitalized dogs in an Australian veterinary referral centre
Creators: Siyu Guo - School of Veterinary Science, University of Queensland, Gatton Campus, QLD 4343, Australia
Huub J M Brouwers
Rowland N Cobbold
Joanne L Platell
Toni A Chapman
Vanessa R Barrs
James R Johnson
Darren J Trott
Publication Details: Journal of antimicrobial chemotherapy, Vol.68(5), pp.1025-1031
Publisher: England
Grant note: I01 CX000192 / CSRD VA
Identifiers: 99900547759701842
Language: English
Resource Type: Journal article