Follicle-stimulating hormone (FSH) regulates the expression of FSH receptor messenger ribonucleic acid in cultured Sertoli cells and in hypophysectomized rat testis

S M Maguire; W A Tribley; M D Griswold

doi:10.1095/biolreprod56.5.1106

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Follicle-stimulating hormone (FSH) regulates the expression of FSH receptor messenger ribonucleic acid in cultured Sertoli cells and in hypophysectomized rat testis

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Follicle-stimulating hormone (FSH) regulates the expression of FSH receptor messenger ribonucleic acid in cultured Sertoli cells and in hypophysectomized rat testis

S M Maguire, W A Tribley and M D Griswold

Biology of reproduction, Vol.56(5), pp.1106-1111

05/1997

DOI: https://doi.org/10.1095/biolreprod56.5.1106

Handle:

https://hdl.handle.net/2376/110273

PMID: 9160707

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Abstract

Sertoli Cells - metabolism

Promoter Regions, Genetic

Follicle Stimulating Hormone - metabolism

Testis - metabolism

Receptors, FSH - genetics

RNA, Messenger - genetics

Cells, Cultured

Rats

Male

RNA, Messenger - metabolism

Testosterone - pharmacology

Rats, Sprague-Dawley

Sertoli Cells - drug effects

Testis - drug effects

DNA - genetics

Follicle Stimulating Hormone - pharmacology

Gene Expression Regulation - drug effects

Animals

Base Sequence

Hypophysectomy

Pituitary Gland - physiology

FSH acts on Sertoli cells via interaction with a transmembrane receptor (FSHr). Control of expression of the receptor is surely a factor in the regulation of the action of FSH. The regulation of FSHr by FSH and testosterone was studied both in culture and in vivo. Sertoli cells from 18- to 20-day-old male rats were cultured in the presence of 25 ng/ml ovine (o) FSH. At 8 h after addition of FSH, expression of FSHr mRNA decreased significantly. Addition of FSH and actinomycin D to cells did not result in a further decrease in FSHr mRNA levels, suggesting that FSH does not alter turnover of FSHr mRNA. Treatment of cells with 40 ng/ml testosterone did not have any significant effect on the expression of FSHr mRNA. Hypophysectomy of 20-day-old male rats resulted in an increase in expression of FSHr mRNA as compared to that in sham-hypophysectomized animals. This increase was measured at 24 h posthypophysectomy and was maintained at 72 h after surgery. Injection of rats with 0.2 U oFSH at 48 h posthypophysectomy resulted in a reduction in FSHr mRNA when compared to the levels in hypophysectomized rats. Treatment with 2 mg testosterone propionate had no effect on FSHr mRNA levels. The findings confirm that FSH plays an important role in regulating mRNA expression of the FSHr in Sertoli cells in culture and show for the first time that FSHr mRNA is regulated in vivo by FSH in the immature rat testis.

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Title: Follicle-stimulating hormone (FSH) regulates the expression of FSH receptor messenger ribonucleic acid in cultured Sertoli cells and in hypophysectomized rat testis
Creators: S M Maguire - Department of Biochemistry and Biophysics, Washington State University, Pullman 99164-4660, USA
W A Tribley
M D Griswold
Publication Details: Biology of reproduction, Vol.56(5), pp.1106-1111
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: HD 10808 / NICHD NIH HHS
Identifiers: 99900547262801842
Language: English
Resource Type: Journal article