Journal article
Follicle-stimulating hormone regulation of A-kinase anchoring proteins in granulosa cells
The Journal of biological chemistry, Vol.268(28), pp.20729-20732
10/05/1993
Handle:
https://hdl.handle.net/2376/109057
PMID: 8407895
Abstract
It has been well established that the biochemical and morphological changes during maturation of granulosa cells that are induced by follicle-stimulating hormone (FSH) occur through the elevation of intracellular cAMP and consequent activation of the cAMP-dependent protein kinase (PKA). In this report we show that FSH action alters the expression of A-Kinase Anchoring Proteins (AKAPs), which function to target the subcellular distribution of the type II PKA. Exposure of granulosa cells grown in primary culture with FSH and estradiol for 72 h resulted in the up-regulation of an 80-kDa AKAP and the RII beta subunit of PKA, whereas cells grown in control medium containing only estradiol produced a time-dependent increase of a 140-kDa AKAP. RII overlays performed with [32P]RII alpha preferentially detected RII-binding bands of 80 and 95 kDa compared to blots probed with [32P]RII beta, suggesting that FSH may alter the subcellular location of PKA in an isoform-specific manner. FSH treatment causes a translocation of RII alpha from the particulate to the cytosolic fraction coincident with the induction of the 80-kDa AKAP, which is also predominately cytosolic. These data suggest that FSH promotes a redistribution of the type II PKA holoenzyme through the selective induction of an RII isoform-specific AKAP.
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Details
- Title
- Follicle-stimulating hormone regulation of A-kinase anchoring proteins in granulosa cells
- Creators
- D W Carr - Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201-3098D A DeMannoA AtwoodM Hunzicker-DunnJ D Scott
- Publication Details
- The Journal of biological chemistry, Vol.268(28), pp.20729-20732
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- United States
- Grant note
- HD 21921 / NICHD NIH HHS GM 48231 / NIGMS NIH HHS DK 44239 / NIDDK NIH HHS
- Identifiers
- 99900547368101842
- Language
- English
- Resource Type
- Journal article