Fourier Transform-Ion Mobility-Orbitrap Mass Spectrometer: A Next-Generation Instrument for Native Mass Spectrometry

Michael L Poltash; Jacob W McCabe; Mehdi Shirzadeh; Arthur Laganowsky; Brian H Clowers; David H Russell

doi:10.1021/acs.analchem.8b02463

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Fourier Transform-Ion Mobility-Orbitrap Mass Spectrometer: A Next-Generation Instrument for Native Mass Spectrometry

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Fourier Transform-Ion Mobility-Orbitrap Mass Spectrometer: A Next-Generation Instrument for Native Mass Spectrometry

Michael L Poltash, Jacob W McCabe, Mehdi Shirzadeh, Arthur Laganowsky, Brian H Clowers and David H Russell

Analytical chemistry (Washington), Vol.90(17), pp.10472-10478

09/04/2018

DOI: https://doi.org/10.1021/acs.analchem.8b02463

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https://hdl.handle.net/2376/106505

PMCID: PMC6464636

PMID: 30091588

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Abstract

A new instrument configuration for native ion mobility-mass spectrometry (IM-MS) is described. Macromolecule ions are generated by using a static ESI source coupled to an RF ion funnel, and these ions are then mobility and mass analyzed using a periodic focusing drift tube IM analyzer and an Orbitrap mass spectrometer. The instrument design retains the capabilities for first-principles determination of rotationally averaged ion-neutral collision cross sections and high-resolution measurements in both mobility and mass analysis modes for intact protein complexes. Operation in the IM mode utilizes FT-IMS modes (originally described by Knorr ( Knorr, F. J. Anal. Chem. 1985, 57(2), 402−406 )), which provides a means to overcome the inherent duty cycle mismatch for drift tube (DT)-IM and Orbitrap mass analysis. The performance of the native ESI-FT-DT-IM-Orbitrap MS instrument was evaluated using the protein complexes Gln K (MW 44 kDa) and streptavidin (MW 53 kDa) bound to small molecules (ADP and biotin, respectively) and transthyretin (MW 56 kDa) bound to thyroxine and zinc.

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Title: Fourier Transform-Ion Mobility-Orbitrap Mass Spectrometer: A Next-Generation Instrument for Native Mass Spectrometry
Creators: Michael L Poltash - Department of Chemistry
Jacob W McCabe - Department of Chemistry
Mehdi Shirzadeh - Department of Chemistry
Arthur Laganowsky - Department of Chemistry
Brian H Clowers - Department of Chemistry
David H Russell - Department of Chemistry
Publication Details: Analytical chemistry (Washington), Vol.90(17), pp.10472-10478
Academic Unit: Chemistry, Department of
Publisher: American Chemical Society
Identifiers: 99900546522401842
Language: English
Resource Type: Journal article