Journal article
Fourier transform infrared spectroscopy, detection and identification of Escherichia coli O157:H7 and Alicyclobacillus strains in apple juice
International journal of food microbiology, Vol.111(1), pp.73-80
2006
Handle:
https://hdl.handle.net/2376/108738
PMID: 16860897
Abstract
Fourier Transform Infrared (FT-IR) spectroscopy (4000–400 cm
−
1
) combined with multivariate statistical methods were used to identify and detect
Escherichia coli O157:H7 from
Alicyclobacillus spp. recovered from apple juice. Four treatments and a control in triplicate experiments (
N
=
3) were studied; the first three treatments of pasteurized apple juice were inoculated with
E. coli O157:H7 ATCC 35150,
Alicyclobacillus acidoterrestris 1016 and
Alicyclobacillus spp. C-Fugi-6 respectively. The fourth treatment was a 1:1 (v:v) mixed culture of both
A.
acidoterrestris 1016 and
Alicyclobacillus spp. C-Fugi-6. The control was uninoculated pasteurized apple juice.
The second derivative transformation and loadings plot over the range of 1800–900 cm
−1 highlighted the most distinctive variations among bacterial spectra. Loadings 1 and 2 were distinctively representative of the bacterial spectral data and accounted for 73% of the total variability. Treatments were noticeably segregated with distinct clustering by principal component analysis (PCA). Using soft independent modeling of class analogy (SIMCA) analysis, 88.3% of (
E.
coli O157:H7 ATCC 35150) spectra, 75.0% of (
A. acidoterrestris 1016) spectra, 88.3% of (
Alicyclobacillus spp. C-Fuji-6) spectra, and 80.0% of the mixed culture of both
Alicyclobacillus strains spectra were correctly classified. Using the spectral features of bacterial cellular constituents such as nucleic acids, proteins, phospholipids, peptidoglycan, and lipopolysaccharides from examined bacterial cells, pure and mixed cultures of
Alicyclobacillus spp. cells, and the pathogenic
E. coli cells could be detected in apple juice.
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Details
- Title
- Fourier transform infrared spectroscopy, detection and identification of Escherichia coli O157:H7 and Alicyclobacillus strains in apple juice
- Creators
- Hamzah M Al-Qadiri - Department of Food Science and Human Nutrition, Box 646376, Washington State University, Pullman, WA 99164-6376, United StatesMengshi Lin - Department of Food Science and Human Nutrition, Box 646376, Washington State University, Pullman, WA 99164-6376, United StatesAnna G Cavinato - Chemistry and Biochemistry Program, Eastern Oregon University, One University Blvd., La Grande, OR 97850-2899, United StatesBarbara A Rasco - Department of Food Science and Human Nutrition, Box 646376, Washington State University, Pullman, WA 99164-6376, United States
- Publication Details
- International journal of food microbiology, Vol.111(1), pp.73-80
- Academic Unit
- Food Science, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547353901842
- Language
- English
- Resource Type
- Journal article