Journal article
Function of FlhB, a Membrane Protein Implicated in the Bacterial Flagellar Type III Secretion System
PloS one, Vol.8(7), pp.e68384-e68384
07/11/2013
Handle:
https://hdl.handle.net/2376/110701
PMCID: PMC3708937
PMID: 23874605
Abstract
The membrane protein FlhB is a highly conserved component of the flagellar secretion system, and it plays an active role in the regulation of protein export. In this study conserved properties of FlhB that are important for its function were investigated. Replacing the
flhB
gene (or part of the gene) in
Salmonella typhimurium
with the
flhB
gene of the distantly related bacterium
Aquifex aeolicus
greatly reduces motility. However, motility can be restored to some extent by spontaneous mutations in the part of
flhB
gene coding for the cytoplasmic domain of
Aquifex
FlhB. Structural analysis suggests that these mutations destabilize the structure. The secondary structure and stability of the mutated cytoplasmic fragments of FlhB have been studied by circular dichroism spectroscopy. The results suggest that conformational flexibility could be important for FlhB function. An extragenic suppressor mutation in the
fliS
gene, which decreases the affinity of FliS to FliC, partially restores motility of the FlhB substitution mutants.
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Details
- Title
- Function of FlhB, a Membrane Protein Implicated in the Bacterial Flagellar Type III Secretion System
- Creators
- Vladimir A MeshcheryakovClive S BarkerAlla S KostyukovaFadel A Samatey
- Publication Details
- PloS one, Vol.8(7), pp.e68384-e68384
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Public Library of Science; San Francisco, USA
- Identifiers
- 99900547189401842
- Language
- English
- Resource Type
- Journal article