Function of the Caenorhabditis elegans ABC transporter PGP-2 in the biogenesis of a lysosome-related fat storage organelle

Lena K Schroeder; Susan Kremer; Maxwell J Kramer; Erin Currie; Elizabeth Kwan; Jennifer L Watts; Andrea L Lawrenson; Greg J Hermann

doi:10.1091/mbc.E06-08-0685

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Function of the Caenorhabditis elegans ABC transporter PGP-2 in the biogenesis of a lysosome-related fat storage organelle

Journal article

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Function of the Caenorhabditis elegans ABC transporter PGP-2 in the biogenesis of a lysosome-related fat storage organelle

Lena K Schroeder, Susan Kremer, Maxwell J Kramer, Erin Currie, Elizabeth Kwan, Jennifer L Watts, Andrea L Lawrenson and Greg J Hermann

Molecular biology of the cell, Vol.18(3), pp.995-1008

03/2007

DOI: https://doi.org/10.1091/mbc.E06-08-0685

Handle:

https://hdl.handle.net/2376/104072

PMCID: PMC1805080

PMID: 17202409

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Abstract

Embryo, Nonmammalian - cytology

Caenorhabditis elegans - metabolism

Caenorhabditis elegans Proteins - chemistry

Adenosine Triphosphatases - metabolism

Caenorhabditis elegans Proteins - metabolism

Exons - genetics

Lipid Metabolism

Mutation - genetics

ATP Binding Cassette Transporter, Sub-Family B - chemistry

Gastrointestinal Tract - metabolism

Adaptor Protein Complex 3

Protein Transport

Endocytosis

Animals

Lysosomes - metabolism

Cytoplasmic Granules - metabolism

ATP Binding Cassette Transporter, Sub-Family B - metabolism

ATP-Binding Cassette Transporters - metabolism

Birefringence

Gastrointestinal Tract - cytology

ATP Binding Cassette Transporter, Sub-Family B - genetics

Caenorhabditis elegans Proteins - genetics

Intracellular Membranes - metabolism

Caenorhabditis elegans gut granules are intestine specific lysosome-related organelles with birefringent and autofluorescent contents. We identified pgp-2, which encodes an ABC transporter, in screens for genes required for the proper formation of gut granules. pgp-2(-) embryos mislocalize birefringent material into the intestinal lumen and are lacking in acidified intestinal V-ATPase-containing compartments. Adults without pgp-2(+) function similarly lack organelles with gut granule characteristics. These cellular phenotypes indicate that pgp-2(-) animals are defective in gut granule biogenesis. Double mutant analysis suggests that pgp-2(+) functions in parallel with the AP-3 adaptor complex during gut granule formation. We find that pgp-2 is expressed in the intestine where it functions in gut granule biogenesis and that PGP-2 localizes to the gut granule membrane. These results support a direct role of an ABC transporter in regulating lysosome biogenesis. Previously, pgp-2(+) activity has been shown to be necessary for the accumulation of Nile Red-stained fat in C. elegans. We show that gut granules are sites of fat storage in C. elegans embryos and adults. Notably, levels of triacylglycerides are relatively normal in animals defective in the formation of gut granules. Our results provide an explanation for the loss of Nile Red-stained fat in pgp-2(-) animals as well as insight into the specialized function of this lysosome-related organelle.

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Title: Function of the Caenorhabditis elegans ABC transporter PGP-2 in the biogenesis of a lysosome-related fat storage organelle
Creators: Lena K Schroeder - Department of Biology, Lewis and Clark College, Portland, OR 97219, USA
Susan Kremer
Maxwell J Kramer
Erin Currie
Elizabeth Kwan
Jennifer L Watts
Andrea L Lawrenson
Greg J Hermann
Publication Details: Molecular biology of the cell, Vol.18(3), pp.995-1008
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: R01 DK074114-02 / NIDDK NIH HHS R01-DK074114 / NIDDK NIH HHS R01 DK074114 / NIDDK NIH HHS
Identifiers: 99900546966201842
Language: English
Resource Type: Journal article