Functional Identification of a Goldfish Odorant Receptor

David J Speca; David M Lin; Peter W Sorensen; Ehud Y Isacoff; John Ngai; Andrew H Dittman

doi:10.1016/S0896-6273(00)80802-8

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Functional Identification of a Goldfish Odorant Receptor

Journal article

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Peer reviewed

Functional Identification of a Goldfish Odorant Receptor

David J Speca, David M Lin, Peter W Sorensen, Ehud Y Isacoff, John Ngai and Andrew H Dittman

Neuron (Cambridge, Mass.), Vol.23(3), pp.487-498

1999

DOI: https://doi.org/10.1016/S0896-6273(00)80802-8

Handle:

https://hdl.handle.net/2376/111562

PMID: 10433261

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https://doi.org/10.1016/S0896-6273(00)80802-8View

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Abstract

The vertebrate olfactory system utilizes odorant receptors to receive and discriminate thousands of different chemical stimuli. An understanding of how these receptors encode information about an odorant's molecular structure requires a characterization of their ligand specificities. We employed an expression cloning strategy to identify a goldfish odorant receptor that is activated by amino acids—potent odorants for fish. Structure–activity analysis indicates that the receptor is preferentially tuned to recognize basic amino acids. The receptor is a member of a multigene family of G protein–coupled receptors, sharing sequence similarities with the calcium sensing, metabotropic glutamate, and V2R class of vomeronasal receptors. The ligand tuning properties of the goldfish amino acid odorant receptor provide information for unraveling the molecular mechanisms underlying olfactory coding.

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Title: Functional Identification of a Goldfish Odorant Receptor
Creators: David J Speca - Department of Molecular and Cell Biology, Division of Neurobiology, University of California, Berkeley, Berkeley, California 94720, USA
David M Lin - Department of Molecular and Cell Biology, Division of Neurobiology, University of California, Berkeley, Berkeley, California 94720, USA
Peter W Sorensen - Department of Fisheries and Wildlife, University of Minnesota, St. Paul, Minnesota 55108, USA
Ehud Y Isacoff - Department of Molecular and Cell Biology, Division of Neurobiology, University of California, Berkeley, Berkeley, California 94720, USA
John Ngai - Department of Molecular and Cell Biology, Division of Neurobiology, University of California, Berkeley, Berkeley, California 94720, USA
Andrew H Dittman - Department of Molecular and Cell Biology, Division of Neurobiology, University of California, Berkeley, Berkeley, California 94720, USA
Publication Details: Neuron (Cambridge, Mass.), Vol.23(3), pp.487-498
Academic Unit: Chemical Engineering and Bioengineering, School of
Publisher: Elsevier Inc
Identifiers: 99900547590701842
Language: English
Resource Type: Journal article