Journal article
Functional and molecular features of the Id4+ germline stem cell population in mouse testes
Genes & development, Vol.28(12), pp.1351-1362
06/15/2014
Handle:
https://hdl.handle.net/2376/108035
PMCID: PMC4066404
PMID: 24939937
Abstract
The maintenance of cycling cell lineages relies on undifferentiated subpopulations consisting of stem and progenitor pools. Features that delineate these cell types are undefined for many lineages, including spermatogenesis, which is supported by an undifferentiated spermatogonial population. Here, we generated a transgenic mouse line in which spermatogonial stem cells are marked by expression of an inhibitor of differentiation 4 (Id4)-green fluorescent protein (Gfp) transgene. We found that Id4-Gfp(+) cells exist primarily as a subset of the type A(single) pool, and their frequency is greatest in neonatal development and then decreases in proportion during establishment of the spermatogenic lineage, eventually comprising ∼ 2% of the undifferentiated spermatogonial population in adulthood. RNA sequencing analysis revealed that expression of 11 and 25 genes is unique for the Id4-Gfp(+)/stem cell and Id4-Gfp(-)/progenitor fractions, respectively. Collectively, these findings provide the first definitive evidence that stem cells exist as a rare subset of the A(single) pool and reveal transcriptome features distinguishing stem cell and progenitor states within the mammalian male germline.
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Details
- Title
- Functional and molecular features of the Id4+ germline stem cell population in mouse testes
- Creators
- Frieda Chan - School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, USAMelissa J Oatley - School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, USAAmy V Kaucher - School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, USAQi-En Yang - School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, USACharles J Bieberich - Department of Biological Sciences, University of Maryland at Baltimore County, Baltimore, Maryland 21250, USACooduvalli S Shashikant - Department of Animal Science, The Pennsylvania State University, University Park, Pennsylvania 16802, USAJon M Oatley - School of Molecular Biosciences, Center for Reproductive Biology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, USA
- Publication Details
- Genes & development, Vol.28(12), pp.1351-1362
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- United States
- Grant note
- HD061665 / NICHD NIH HHS R01 HD061665 / NICHD NIH HHS
- Identifiers
- 99900546757301842
- Language
- English
- Resource Type
- Journal article