Journal article
Functional genomics of cell elongation in developing cotton fibers
Plant molecular biology, Vol.54(6), pp.911-929
04/2004
Handle:
https://hdl.handle.net/2376/106782
PMID: 15604659
Abstract
Cotton fibers are single-celled seed trichomes of major economic importance. Factors that regulate the rate and duration of cell expansion control fiber morphology and important agronomic traits. For genetic characterization of rapid cell elongation in cotton fibers, ∼ 14,000 unique genes were assembled from 46,603 expressed sequence tags (ESTs) from developmentally staged fiber cDNAs of a cultivated diploid species (Gossypium arboreumL.). Conservatively, the fiber transcriptome represents 35–40% of the genes in the cotton genome. In silico expression analysis revealed that rapidly elongating fiber cells exhibit significant metabolic activity, with the bulk of gene transcripts, represented by three major functional groups – cell wall structure and biogenesis, the cytoskeleton and energy/carbohydrate metabolism. Oligonucleotide microarrays revealed dynamic changes in gene expression between primary and secondary cell wall biogenesis showing that fiber genes in the dbEST are highly stage-specific for cell expansion – a conclusion supported by the absence of known secondary cell wall-specific genes from our fiber dbEST. During the developmental switch from primary to secondary cell wall syntheses, 2553 “expansion-associated” fiber genes are significantly down regulated. Genes (81) significantly up-regulated during secondary cell wall synthesis are involved in cell wall biogenesis and energy/carbohydrate metabolism, which is consistent with the stage of cellulose synthesis during secondary cell wall modification in developing fibers. This work provides the first in-depth view of the genetic complexity of the transcriptome of an expanding cell, and lays the groundwork for studying fundamental biological processes in plant biology with applications in agricultural biotechnology.
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Details
- Title
- Functional genomics of cell elongation in developing cotton fibers
- Creators
- A Arpat - Department of Agronomy and Range Science University of California One Shields Ave. CA USAMark Waugh - National Center for Genome Resources 2935 Rodeo Park Drive East NM USAJohn Sullivan - National Center for Genome Resources 2935 Rodeo Park Drive East NM USAMichael Gonzales - National Center for Genome Resources 2935 Rodeo Park Drive East NM USADavid Frisch - Genome Center of Wisconsin University of Wisconsin 445 Henry Mall, Genetics Bldg. B19 WI USADorrie Main - Computer Science Department University of Massachusetts Massachusetts USATodd Wood - Center for Origins Research and Education Bryan College TN USAAnna Leslie - College of Agriculture and Environmental Sciences Genomics Facility University of California One Shields Ave CA USARod Wing - Arizona Genomics Institute, Department of Plant Sciences University of Arizona 303 Forbes Bldg AZ USAThea Wilkins - Department of Agronomy and Range Science University of California One Shields Ave. CA USA
- Publication Details
- Plant molecular biology, Vol.54(6), pp.911-929
- Academic Unit
- Horticulture, Department of
- Publisher
- Kluwer Academic Publishers; Dordrecht
- Identifiers
- 99900546608401842
- Language
- English
- Resource Type
- Journal article