Functional prostate-specific membrane antigen is enriched in exosomes from prostate cancer cells

Tiancheng Liu; Desiree E Mendes; Clifford E Berkman

doi:10.3892/ijo.2014.2256

Back

Functional prostate-specific membrane antigen is enriched in exosomes from prostate cancer cells

Journal article

Open access

Peer reviewed

Functional prostate-specific membrane antigen is enriched in exosomes from prostate cancer cells

Tiancheng Liu, Desiree E Mendes and Clifford E Berkman

International journal of oncology, Vol.44(3), pp.918-922

03/2014

DOI: https://doi.org/10.3892/ijo.2014.2256

Handle:

https://hdl.handle.net/2376/102145

PMCID: PMC3928468

PMID: 24424840

Files and links (1)

url

https://doi.org/10.3892/ijo.2014.2256View

Published (Version of record) Open

Abstract

Prostatic Neoplasms - pathology

Endosomal Sorting Complexes Required for Transport - biosynthesis

Prognosis

Humans

Antigens, Surface - biosynthesis

Antigens, Surface - genetics

Exosomes - genetics

Male

Transcription Factors - biosynthesis

Cell Membrane - ultrastructure

Prostatic Neoplasms - diagnosis

Glutamate Carboxypeptidase II - biosynthesis

Microscopy, Electron

Glutamate Carboxypeptidase II - genetics

Prostatic Neoplasms - genetics

Cell Line, Tumor

Biomarkers, Tumor - genetics

Cell Membrane - metabolism

DNA-Binding Proteins - biosynthesis

Biomarkers, Tumor - biosynthesis

Exosomes - ultrastructure

Tetraspanin-29 - biosynthesis

Developing simple and effective approaches to detect tumor markers will be critical for early diagnosis or prognostic evaluation of prostate cancer treatment. Prostate‑specific membrane antigen (PSMA) has been validated as an important tumor marker for prostate cancer progression including angiogenesis and metastasis. As a type II membrane protein, PSMA can be constitutively internalized from the cell surface into endosomes. Early endosomes can fuse with multivesicular bodies (MVB) to form and secrete exosomes (40-100 nm) into the extracellular environment. Herein, we tested whether some of the endosomal PSMA could be transferred to exosomes as an extracellular resource for PSMA. Using PSMA-positive LNCaP cells, the secreted exosomes were collected and isolated from the cultured media. The vesicular structures of exosomes were identified by electron microscopy, and exosomal marker protein CD9 and tumor susceptibility gene (TSG 101) were confirmed by western blot analysis. Our present data demonstrate that PSMA can be enriched in exosomes, exhibiting a higher content of glycosylation and partial proteolysis in comparison to cellular PSMA. An in vitro enzyme assay further confirmed that exosomal PSMA retains functional enzymatic activity. Therefore, our data may suggest a new role for PSMA in prostate cancer progression, and provide opportunities for developing non-invasive approaches for diagnosis or prognosis of prostate cancer.

Metrics

9 Record Views

See more details

Details

Title: Functional prostate-specific membrane antigen is enriched in exosomes from prostate cancer cells
Creators: Tiancheng Liu - Department of Chemistry, Washington State University, Pullman, WA 99164, USA
Desiree E Mendes - Department of Chemistry, Washington State University, Pullman, WA 99164, USA
Clifford E Berkman - Department of Chemistry, Washington State University, Pullman, WA 99164, USA
Publication Details: International journal of oncology, Vol.44(3), pp.918-922
Academic Unit: Chemistry, Department of
Publisher: Greece
Grant note: R01CA140617 / NCI NIH HHS R01 CA140617 / NCI NIH HHS T32 GM008336 / NIGMS NIH HHS
Identifiers: 99900546553501842
Language: English
Resource Type: Journal article