Journal article
Gamma-irradiation and doxorubicin treatment of normal human cells cause cell cycle arrest via different pathways
Molecules and cells, Vol.20(3), pp.331-338
12/31/2005
Handle:
https://hdl.handle.net/2376/102670
PMID: 16404146
Abstract
Ionizing radiation and doxorubicin both produce oxidative damage and double-strand breaks in DNA. Double-strand breaks and oxidative damage are highly toxic and cause cell cycle arrest, provoking DNA repair and apoptosis in cancer cell lines. To investigate the response of normal human cells to agents causing oxidative damage, we monitored alterations in gene expression in F65 normal human fibroblasts. Treatment with g-irradiation and doxorubicin altered the expression of 23 and 68 known genes, respectively, with no genes in common. Both agents altered the expression of genes involved in cell cycle arrest, and arrested the treated cells in G2/M phase 12 h after treatment. 24 h after g-irradiation, the percentage of G1 cells increased, whereas after doxorubicin treatment the percentage of G2/M cells remained constant for 24 h. Our results suggest that F65 cells respond differently to g-irradiation- and doxorubicin-induced DNA damage, probably using entirely different biochemical pathways.
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Details
- Title
- Gamma-irradiation and doxorubicin treatment of normal human cells cause cell cycle arrest via different pathways
- Creators
- Seong Min Lee - School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, KoreaBuHyun YounCha Soon KimChong Soon KimChulHee KangJoon Kim
- Publication Details
- Molecules and cells, Vol.20(3), pp.331-338
- Academic Unit
- Chemistry, Department of
- Publisher
- Korea (South)
- Identifiers
- 99900546531301842
- Language
- English
- Resource Type
- Journal article