Journal article
Gene Expression in the Efferent ducts, Epididymis, and Vas deferens during Embryonic Development of the Mouse
Developmental dynamics, Vol.239(9), pp.2479-2491
09/2010
Handle:
https://hdl.handle.net/2376/107114
PMCID: PMC2939230
PMID: 20652947
Abstract
The tissues of the male reproductive tract are characterized by distinct morphologies, from highly coiled to un-coiled. Global gene expression profiles of efferent ducts, epididymis, and vas deferens were generated from embryonic day 14.5 to postnatal day 1 as tissue-specific morphologies emerge. Expression of homeobox genes, potential mediators of tissue-specific morphological development, was assessed. Twenty homeobox genes were identified as either tissue-enriched, developmentally regulated, or both. Additionally, ontology analysis demonstrated cell adhesion to be highly regulated along the length of the reproductive tract. Regulators of cell adhesion with variable expression between the three tissues were identified including
Alcam
, various cadherins, and multiple integrins. Immunofluorescence localization of the cell adhesion regulators POSTN and CDH2 demonstrated cell adhesion in the epithelium and mesenchyme of the epididymis may change throughout development. These results imply cell adhesion may be modulated in a tissue-specific manner, playing an important role in establishing each tissue’s final morphology.
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Details
- Title
- Gene Expression in the Efferent ducts, Epididymis, and Vas deferens during Embryonic Development of the Mouse
- Creators
- Elizabeth M Snyder - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520Christopher L Small - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520Daniela Bomgardner - Department of Cell Biology, Box 800732, School of Medicine, University of Virginia Health System, Charlottesville, VA 22908Bingfang Xu - Department of Cell Biology, Box 800732, School of Medicine, University of Virginia Health System, Charlottesville, VA 22908Ryan Evanoff - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520Michael D Griswold - Center for Reproductive Biology, School of Molecular Biosciences, Washington State University, Pullman, WA 99164-7520Barry T Hinton - Department of Cell Biology, Box 800732, School of Medicine, University of Virginia Health System, Charlottesville, VA 22908
- Publication Details
- Developmental dynamics, Vol.239(9), pp.2479-2491
- Academic Unit
- Molecular Biosciences, School of
- Identifiers
- 99900546873501842
- Language
- English
- Resource Type
- Journal article