Journal article
Gene Expression in the Rat Brain During Prostaglandin D2- and Adenosinergically-Induced Sleep
Journal of neurochemistry, Vol.105(4), pp.1480-1498
05/2008
Handle:
https://hdl.handle.net/2376/104591
PMCID: PMC2590502
PMID: 18331290
Abstract
Previous studies have supported the hypothesis that macromolecular synthesis occurs in the brain during sleep as a response to prior waking activities and that prostaglandin D
2
(PGD
2
) is an endogenous sleep substance whose effects are dependent on adenosine A
2a
receptor-mediated signaling. We compared gene expression in the cerebral cortex, basal forebrain and hypothalamus during PGD
2
-induced and adenosinergically-induced sleep to results from our previously-published study of recovery sleep (RS) after sleep deprivation (SD). Immediate early gene (IEG) expression in the cortex during sleep induced by PGD
2
- or by the selective adenosine A
2a
agonist CGS21680 showed limited similarity to that observed during RS while, in the basal forebrain and hypothalamus, widespread activation of IEGs not seen during RS occurred. In all three brain regions, PGD
2
and CGS21680 reduced the expression of
arc
, a transcript whose expression is elevated during SD. Using GeneChips
®
, the majority of genes induced by either PGD
2
or CGS21680 were induced by both, suggesting activation of the same pathways. However, gene expression induced in the brain after PGD
2
or CGS21680 treatment was distinct from that described during RS after SD and apparently involves glial cell gene activation and signaling pathways in neural-immune interactions.
Metrics
5 Record Views
Details
- Title
- Gene Expression in the Rat Brain During Prostaglandin D2- and Adenosinergically-Induced Sleep
- Creators
- Akira Terao - Biosciences Division, SRI International, Menlo Park, CA 94305Zhi-Li Huang - Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Suita, Osaka, 565-0874, JapanJonathan P Wisor - Biosciences Division, SRI International, Menlo Park, CA 94305Takatoshi Mochizuki - Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Suita, Osaka, 565-0874, JapanDmitry Gerashchenko - Biosciences Division, SRI International, Menlo Park, CA 94305Yoshihiro Urade - Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Suita, Osaka, 565-0874, JapanT.S Kilduff - Biosciences Division, SRI International, Menlo Park, CA 94305
- Publication Details
- Journal of neurochemistry, Vol.105(4), pp.1480-1498
- Academic Unit
- Biomedical Sciences, Department of
- Identifiers
- 99900546889401842
- Language
- English
- Resource Type
- Journal article