Journal article
Gene expression analysis distinguishes tissue specific and gender related functions among adult Ascaris suum tissues
Molecular genetics and genomics : MGG, Vol.288(5), pp.243-260
06/2013
Handle:
https://hdl.handle.net/2376/107379
PMCID: PMC3670759
PMID: 23572074
Abstract
Over a billion people are infected by
Ascaris
spp. intestinal parasites. To clarify functional differences among tissues of adult A. suum, we compared gene expression by various tissues of these worms by expression microarray methods.. The
A. suum
genome was sequenced and assembled to allow generation of microarray elements. Expression of over 40,000 60-mer elements was investigated in a variety of tissues from both male and female adult worms. Nearly 50 percent of the elements for which signal was detected exhibited differential expression among different tissues. The unique profile of transcripts identified for each tissue clarified functional distinctions among tissues, such as chitin binding in the ovary and peptidase activity in the intestines. Interestingly, hundreds of gender-specific elements were characterized in multiple non-reproductive tissues of female or male worms, with most prominence of gender differences in intestinal tissue.
A. suum
genes from the same family were frequently expressed differently among tissues. Transcript abundance for genes specific to
A. suum
, by comparison to
Caenorhabditis elegans
, varied to a greater extent among tissues than for genes conserved between
A. suum
and
C. elegans
. Analysis using
C. elegans
protein interaction data identified functional modules conserved between these two nematodes, resulting in identification of functional predictions of essential subnetworks of protein interactions and how these networks may vary among nematode tissues. A notable finding was very high module similarity between adult reproductive tissues and intestine. Our results provide the most comprehensive assessment of gene expression among tissues of a parasitic nematode to date.
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Details
- Title
- Gene expression analysis distinguishes tissue specific and gender related functions among adult Ascaris suum tissues
- Creators
- Zhengyuan Wang - The Genome Institute, Washington University School of Medicine, St. Louis, Missouri 63108Xin Gao - The Genome Institute, Washington University School of Medicine, St. Louis, Missouri 63108John Martin - The Genome Institute, Washington University School of Medicine, St. Louis, Missouri 63108Yong Yin - The Genome Institute, Washington University School of Medicine, St. Louis, Missouri 63108Sahar Abubucker - The Genome Institute, Washington University School of Medicine, St. Louis, Missouri 63108Amy C Rash - Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110Ben-Wen Li - Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110Bill Nash - The Genome Institute, Washington University School of Medicine, St. Louis, Missouri 63108Kym Hallsworth-Pepin - The Genome Institute, Washington University School of Medicine, St. Louis, Missouri 63108Douglas P Jasmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Makedonka Mitreva - The Genome Institute, Washington University School of Medicine, St. Louis, Missouri 63108
- Publication Details
- Molecular genetics and genomics : MGG, Vol.288(5), pp.243-260
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Grant note
- R01 GM097435 || GM / National Institute of General Medical Sciences : NIGMS R01 AI081803 || AI / National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID
- Identifiers
- 99900547295301842
- Language
- English
- Resource Type
- Journal article